chromosome isolated 中文意思是什麼
-
Chromosome numbers were no remarkable difference with the control. analysis of isoenzyme ( peroxidase, cytochrome oxidase, esterase ) and rapd indicated that the regenerated plants from protocalli were different with the control. protoplasts were isolated from agrobacterium rhizogenes a ^ transformed cell line of medicago sativa l.
染色體檢查，同工酶（過氧化物酶、細胞色素氧化酶、酯酶）和rapd分析表明，來源於抗性系原生質體的再生植株具一定的遺傳穩定性，但與野生型相比亦發生了一些變異。 -
With high concentration of sds and elevated temprature （ 43 ） ， we succeeded in eliminating plasmids from ten strains of s. aureus which were isolated from the farms of beijing suburb. according to the sensitivity test before and after plasmid remoral ， we found that 72. 5 antibiotic resistance of s. aureus was mediated by plasmids ， and 27. 5 encoded by chromosome. the curing test indicated that the plasmids play an important role in coding the resistance to antibiotics
從北京郊區雞場分離到10株耐藥性金黃色葡萄球菌，對這些菌株進行質粒消除，根據質粒消除前後的耐藥性檢測，發現耐各種藥物的抗性基因大多數位於質粒上，少部分抗性基因位於染色體上，由質粒介導的耐藥基因佔72 . 5 % ，由染色體編碼的抗性基因佔27 . 5 % ，說明質粒在決定對抗生素的抵抗性中起主要作用。 -
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
分享友人