clones 中文意思是什麼

Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。

Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。

After 10 to 15 years of clonal testing, mixture of 25 to 50 clones, often belonging to several families, have been released for commercial use.

經過1015年無性系測定后，常常將屬于幾個家系的2550個無性系混合起來，大量投入生產使用。

There are obvious differences between clones with regard to their self-fertility.

自交能孕性在不同的無性系中是明顯不同的。

Potentially "high-yielding" clones are likely to be the material which first come to hand.

有「高產」可能的無性系大概首先是隨手可得的材料。

They have found that 0. 1～1. 4%of the minicells survive and give rise to clones of cell.

他們發現小細胞中約有0.11.4%能存活並能形成細胞集落。

A compromise has to be made as to the choice of clones to be used as pollen parents.

對于用作授粉親本的一些無性系的選擇，不得不採用一種折衷辦法。

The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %

結果：其中一對引物擴增得到一600bp產物；另一對引物擴增得到三條特異性的dna條帶，大小分別為1 . 5kb 、 1 . 3kb和800bp ，將600bp和800bpdna進行克隆及測序，並推導編碼的氨基酸序列。

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。

Such notable professionals as john rivers hybridized various tree fruits, and introduced superior clones from france and elsewhere.

著名的專業工作者如JohnRivers對各種果樹進行了雜交，並從法國和其他地方引進優良的無性系。

Clonal analysis in this study on the eye imaginal disc showed that in the oxt mutant clones, the ci expression was completely deleted

胚胎分析結果表明則t基因的突變會導致wg的轉錄和engrailed基因的表達受抑。

Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function

進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。

In this paper, genetic transformation systems by agrobacterhim rhizogems was established and optimized and high quality regenerating system was selected. the products of indigotin and indirubin in regenerating plantlets had been detected by hplc. the main studies and results were reported as follows : 1 establishment and selection of hairy root clones taken cotyledons of autotetraploid indigoblue woad as explants and hairy roots induced successfully from them by agrobaterium rhizogenes strains a4 and r1000

本文利用發根農桿菌rit - dna建立並優化了四倍體菘藍的遺傳轉化體系，並對再生植株的主要代謝產物靛藍、靛玉紅進行了含量檢測，篩選了優良無性系，也為實現外源抗蟲、抗病等基因的導入奠定了基礎。

These are replicated individually in bacterial cells to produce a library of different dna clones.

它們各自在細菌細胞內復制，產生不同的DNA克隆的「文庫」。

" either female komodo dragons are spermthecal, meaning that they have the ability to store sperm or fertilised eggs for long periods, in this case for two years, or they are parthogenetic, meaning that they are self - reproductive - they produce clones of themselves, " de la panouse said

其一，每隻雌性科莫多巨蜥都有受精囊，這意味著它們有能力在很長一段時間存儲精子或受精卵。由此看來， sungai可能就將精子或受精卵存儲了兩年之久。

Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。

Sp2 / 0 myeloma cells are fused with spleen cells of balb / c mice ( the ratio is 1 : 10 ) immunized with at - by routine method. positive clones are detected by elisa

Sp2 / 0細胞和免疫脾細胞按1 ： 10比例融合，融合劑peg分子量為1500 ，陽性克隆篩選採用三步進行。

Regional test of poplar clones in section aigeiros

黑楊派無性系區域化試驗初報

Attack of the clones was just a prologue

克隆人的進攻（星球大戰第二集）只是一個序曲。