cloning of n gene 中文意思是什麼

cloning of n gene 解釋
n基因的克隆
  • cloning : 基因轉殖 克隆
  • of : OF ＝Old French 古法語。
  • n : 1. 【羅馬數字】90〈N＝90000〉。2. 【化學】＝nitrogen. 3. ＝North（ern）。N ＝nuclear 核的：N-waste 核廢料。
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique

    本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。
  2. Cloning of the glyphosate n - acetyltransferase gene from soil total dna and its characterization of enzyme activity

    乙酰轉移酶基因的克隆及酶學特性分析
  3. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  4. Not only sequence information from the c - terminus of proteins and peptides is of especial interest in the investigation of n - terminally blocked proteins and peptides, but also c - terminal sequence analysis can facilitate the production of more specific probes for gene cloning

    C端與n端一樣，在蛋白質分子結構分析中具有重要的地位，對其順序測定具有重要的意義，不僅可以對n端封閉的蛋白質進行序列測定，而且對基因克隆有指導意義。
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