competent product 中文意思是什麼
competent product
解釋
競爭產品-
Our knowledge of current market price developments is constantly updated and used in a confidential, competent and professional manner for your benefit. we assist and support our suppliers and customers in product and process developments and improvements
我們既內行又專業的應用市場和價格發展的最新知識，我們幫助客戶和供應商推動他們的研發，我們能迅速又靈活的解決鑄造廠個別的需求。 -
Article 23 where the product of an enterprise fails to reach the state - stipulated standards, the competent authoritative department of the said enterprise shall order the enterprise to rectify production within a fixed period
第二十三條企業產品質量達不到國家規定的標準，企業主管機關應令其限期整頓。 -
Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。 -
The company strives for competitiveness through quality, aims at development through technololgy and seek better performance through management, advocating the corporate culture of humanity priority and respect for talent. the maximum advantage of the company lies in the competent and experienced managerial staff as well as the dedicated and high - caliber team of professionals. in the meantime, the company strives to implement the iso9000 standards to reduce energy consumption and improve product quality
公司堅持以質量求生存，以科技求發展，以管理求效益，倡導「以人為本尊賢重仕」的企業文化。公司最大的優勢源自堅強有力，有著豐富經驗的管理團隊，及具有奉獻精神專業素質的人才。同時，大力推行iso9000系列標準，不斷降低能耗，提升產品質量。 -
First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a
用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。 -
I am a competent product sales representative and promoter
我是個能幹的產品銷售代表及推銷員。 -
Cloning and analyzing of the full - length cdna sequence the fragments consistent with expected length from the product of race - pcr reaction are separated, purified and recovered, after which the fragments are cloned into the vector of pmd - 1st to transform the competent cells
山na的克隆和測序將得到的3 』一及5 』 scepcr產物符合預期長度的片段，分離后純化、回收，克隆到ta克隆載體pmd 18t ，轉化e -
The organization shall select and assign personnel to ensure that those whose activies impact the conformity of product and / or service are competent on the basis of applicable education, training and experience
凡是其工作會影響的產品和/或服務符合性的各類工作人員，組織必須根據相應的教育、培訓和經驗要求去選擇並委派這些工作人員，以確保他（她）們能夠勝任工作。 -
In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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