competitive binding 中文意思是什麼

competitive binding 解釋
競爭結合,競爭性結合
  • competitive : adj 競爭的，競賽的。 competitive bidding system 招標制。 competitive examination 競爭考試。 compe...
  • binding : adj 1 縛[捆、綁]…的；黏合的；系連的，連結的。2 有束縛力的，有拘束力的，附有義務的。3 〈口語〉引起...
  1. A competitive binding assay used to determine hapten iaa with the aid of hrp - iaa conjugate has been developed. it was based on the ordered, stable mercapto self - assembled monolayer formed on gold surface, which immobilized igg with covalent bond

    方法基於金基底上形成的均一、穩定、有序的巰基乙酸自組裝單層膜，在edc 、 nhs活化下，能以共價方式固定抗體。
  2. Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen

    El舊a拐成惴現2個克隆的可容牲mg7seem明顯的抗原結合舌隊其a分別為0 832和0 912 ，均高出附性對照（ 0
  3. Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

    X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。
  4. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  5. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  6. Subsequently, the binding of ( 35 ) ~ s - cam and a. thaliana protoplasts were processed, including association, dissociation, competitive and saturation analysis. the results show the binding of ( 35 ) ~ s - cam and protoplasts is rapid, reversible, saturable and sensitive to proteinase ; otherwise the specificity of binding was assessed with different competitors. k _ ( d ) of 9. 2 nm and about 25, 000 binding sites per protoplast were gained through saturation analysis

    隨后，對~ （ 35 ） s - cam和擬南芥原生質體的結合進行了受體學分析，包括結合、解離、競爭性和飽和分析，結果顯示~ （ 35 ） s - cam和擬南芥原生質體的結合具有結合快速性、可逆性、競爭特異性和濃度飽和性，從飽和分析可以得出k _ d約為9 . 2nm ，每個原生質體表面大約含有25 ， 000個結合位點，並且其結合對蛋白酶敏感。
  7. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  8. As the same time, mianyang telecom combine the chinatelecom group market stratagem and local competitive situation, selecting an aimed clients group, according to clients described characters, adopting different product binding and price analysis. therefore, the aim clients are interested in the scheme that is designed

    以固定電話和小靈通為代表的傳統語音業務日趨萎縮， 「增量不增收」 、 「存量流失嚴重，增量不明顯」等現象日益凸現，對客戶不了解越來越成為語音業務營銷工作的難點和短板。
  9. Through three rounds of screening, seventeen clones were selected and used in competitive test. the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera. based on the amin acid sequences deduced from the foreign sequence inserted in the phage, it was indicated that all clones shared the core sequence - p / ekphf, that was similar to aa50 ~ aa55 domain of n protein of prrsv

    從第三輪親和篩選的噬菌體中隨機挑取17個克隆進行功能鑒定，結果表明8個克隆與mabge3具有較強的特異性結合力並可以被prrsv陽性血清阻斷，測序發現7個克隆具有核心序列： p ekphf ，該序列與prrsvn蛋白aa50 aa55 （ p ekphf ）具有較高的同源性。
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