cona 中文意思是什麼

cona 解釋
措娜
  1. Preventive effect of ifn - receptor - immunoglobulin fusion protein on liver demages induced by cona in mice

    誘導小鼠肝損傷的保護作用
  2. In order to attain bioactive glycoprotein, glycoprotein of the leaves of camellia sinensis, was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography, by cona - sepharose 4b affinity chromatography

    為了純化天然糖蛋白，進行了茶樹葉糖蛋白的cona - sepharose4b親和層析，從sephadexg - 100凝膠過濾收集的粗糖蛋白中，分離茶樹葉糖蛋白。
  3. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  4. Methods subacute senile mouse model was established by injection of d - galactose. the splenic cells were isolated from mice and cultured in vitro following cona stimulation. expression of cd 137 on splenic t cells in the model and normal control groups were compared with each other by flow cytometry method at various cultural times after cona stimulation

    方法取5周齡balb c雄性小鼠，頸背部皮下注射d -半乳糖，分別于造模型后一到五個月取脾臟淋巴細胞，同時取自然衰老小鼠脾臟淋巴細胞，在培養的第0 、 24 、 48 、 72 、 96 、 120 、 144h收集細胞，用流式細胞儀分析脾臟t細胞cd137的表達陽性率。
  5. Fty were also found to inhibit the expression of cd25 on t cells stimulated by cona. conclusion : we assumed tolerance to allograft was induced by and only by the pretreatment of fty in this stuty

    結論：我們的實驗完整觀察了fty對淋巴細胞及t細胞亞群的影響，驗證了fty可誘導淋巴細胞凋亡，首次發現fty對t細胞早期活化反應的抑制作用，其中包括減少cd25的表達。
  6. 3. the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme, the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona. the results showed that lymphocyte proliferation induced by pha was better than by cona. 4

    3 、 gpif免疫生物活性評價方法的優化對gpife玫瑰花環實驗在不影響srbc活性的基礎上用神經氨酸酶處理，經此法處理后e玫瑰花環形成率明顯提高；對傳統的cona誘導的人外周血淋巴細胞增殖brdu - elisa實驗，進行了方法學改進。
  7. According to published chil - 15 gene sequence, a pair of primers were designed and synthesized to clone chil - 15 cdna from cona - activated chicken splenocytes by rt - pcr. recombinant plasmid puc19chil - 15 carrying the chil - 15 gene was constructed

    雞il - 15 （ chil - 15 ）是一種新發現的且與il - 2活性相似的細胞因子，作為疫苗佐劑研究的重要候選細胞因子有巨大潛力。
  8. Further, in the mouse spleen t lymphocytes activation system, it was found that, without activation by cona, there were no or extremely low level of expression of baf complex and nf1 / ctf in the spleen t lymphocytes

    進一步用小鼠t淋巴細胞活化體系的研究發現，未經cona激活的t淋巴細胞中， baf復合物和轉錄因子nf1 ctf不表達或低水平表達。
  9. Interferon - is an antiviral cytokine produced by activated t cell and nk cell. it upregulated mhc ii antigen expression, influenced the cellular and humoral immune response and has pleiotropic regulatory effects on immune system. one gene was amplified by reverse transcription - polymerase chain reaction ( rt - pcr ) in splenocytes which was stimulated with cona for 24 hours

    -干擾素（ interferon - ， ifn - ）主要由活化的t細胞及nk細胞產生，具有抗病毒作用，也具有上調mhc （ majorhistocompatibilitycomplex ， mhc ）類抗原分子的表達等作用，是影響機體細胞免疫和體液免疫反應的一類具有多種調節效應的細胞因子。
  10. Basing on these backgrounds and motives, the cona expressing library of plasmodium falciparum from hainan was constructed

    正是基於以上目的和研究背景，我們構建了惡性瘧原蟲海南株紅內期cdna表達文庫。
  11. After activated by cona, the mouse spleen t lymphocytes started expressing baf complex and nf1 / ctf. the longer the stimulation, the higher the expression level of baf complex and nf1 / ctf in the cells

    經cona激活后，小鼠脾t淋巴細胞中baf復合物和轉錄因子nf1 ctf開始表達，隨著刺激時間的增加，表達量增加。
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