conserved domain 中文意思是什麼
conserved domain
解釋
保存區段-
The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii
並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。 -
The sequence analysis indicated that eight of the nine analogues contained the conserved motifs of nbs - lrr of disease resistant genes, such as p - loop ( kinase la ), kinase 2, kinase 3a and trans - membrane domain. the sequence of amino acid of clone cr271 was highly homologic ( 61 % identify and 76 % similarity ) to blight resistance gene xal of rice to xanthomonas oryzae pv
然後進行了序列的測定，通過全序列分析，結果表明：所獲得的8個抗病基因同源序列均含有nbs - lrr類抗病基因的保守序列，如p - loop （ kinase1a ） 、 kinase2 、 kinase3a以及跨膜結構域等。 -
The residues capable of forming amphipathic a - helice structure in the first conserved domain are identical to those of ap2, while the structure in the second conserved domain lacks 9 amino acids as compared to that of ap2
螺旋的氨基酸殘基與apz的完全相同，而另一個區域中形成。螺旋g ：氦基酸歿基與apz相比少9個氨基酸。 -
The conserved cystein residues also present in the cub domain of clsp at position of 36, 94 and 112
Rt一pcr分析結果證實clspmrna在dc中存在一種時間依賴性的表達模式。 -
The predicated amino acid sequences contain two conserved functional domains : a dna - binding domain ( hmg - box ) and a transactivation domain. the conserved motif of group b homology lies immediate adjacent to c - proximal region of the hmg - box. similar structure and identity of sox2 gene among mammals, birds, amphibians and reptiles suggest that sox2 gene have evolutionary conserved roles
進一步分析表明，已巴灘2編碼的238個氨基酸多膚鏈含有兩個保守的功能區域:位於n端的hmg盒區（ 1一78aa ）和位於c端的轉錄激活區（ 131一236aa ） ，以及一個保守的特徵性b亞族同源區域（ 79一90aa ）與hmg盒區的c端相連。 -
A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf
根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。 -
The comparative analysis of sequences indicated that the sequences of meq in different pathotypes are relatively conserved and the homology of the amino acid sequences is very high. the significant differences include two mutations in both mdv - 1 vaccine strains cvi988 / rispens and 814 strain : the deletion of a proline ( no. l93aa ), and this mutation is just exactly located in a 15 - amino - acid ( eelcaqlcstppppi ) repeat sequence within the c - terminal transactivation domain of meq protein ; and a point mutation with a shift from alanine ( a ) of all virulent strains to serine ( s ) was occurred on the no. 71 aa
結果發現， mdv不同致病型的meq基因序列相對比較保守，它們相互間核苷酸和氨基酸序列的同源性均很高；但是，與所有七個致瘤性的mdv毒株相比，二個型弱毒疫苗cv1988 rispens株和814株均出現了兩個特徵性的突變：即第194位的p缺失性突變和第71位氨基酸由a變成了s的位點突變；缺失性突變恰恰位於meq基因中轉錄激活域內的一個多脯氨酸的重復序列（ pppp ）之中。 -
At the n - terminal end is the variable domain of 26 amino acids in length, which has no conserved mgxxc ( s / q ) xxt sequence essential for n - myristoylation. so it is conferred that the vfcpkl is a soluble protein
N端的可變區有26個氨基酸殘基，沒有豆蔻酰化所必需的保守序列mgxxc （ s q ） xxt ，因此推測vfcpk1可能是水溶性蛋白。 -
Notch proteins are a family of evolutionarily conserved transmembrane receptors which are involved in cell fate determination of many cell lineages. notch ligand delta is also transmembrane protein with characteristic cysteine - rich n - terminal domain responsible for notch binding
Notch基因廣泛表達于胚胎發育過程中的各種組織和各種成熟組織中具有增殖能力的細胞層，該基因編碼一個由2753個氨基酸殘基的型跨膜蛋白分子受體，其配體也是細胞膜蛋白，稱為delta 。 -
According to the conserved domain of the pal gene family, we achieved to the segment of pal gene about 950bp in rhodiola crenulata. some proposals were put forward for further development of the important enzymes in the metabolic pathway of salidroside
我們還根據苯丙氨酸解氨酶（ pal ）基因家族的保守序列，以大花紅景天dna為模板，通過pcr擴增得到其約950bp長的pal基因的克隆，測序並比較了它與其基因家族的同源性。 -
The kinase catalytic domain is 264 amino acids in length and contains all of 11 conserved subdomains and 15 invariant amino acid residues essential fo r eukaryotic serine / threonine protein kinases
激酶區長264個氨基酸殘基，具有所有11個保守的亞結構域和15個對于真核生物絲氨酸蘇氨酸蛋白激酶所必需的保守的氨基酸殘基。 -
At the c - terminal end is a calmodulin - like domain of 162 amino acids in length, which has four highly conserved ca2 + - binding ef hands
C端的調節區有162個氨基酸殘基，有4個非常保守的ef手性鈣結合結構域。 -
( 3 ) 490bp cdna fragment of fe ( ii ) - transporter gene mxlrtl was cloned by using common pcr method from iron - stressed root cdna library of malus xiaojinensis cheng et jiang with primers designed according to the conserved domain sequences of plant irt gene families. then we cloned the full cdna of mxlrtl gene by race method from this library with primers designed according to the sequence of 490bp cdna fragment
（ 3 ）根據植物irt基因家族的功能保守區序列設計引物，首先通過常規pcr法從缺鐵脅迫處理的小金海棠根系cdna文庫中克隆了fe （ ） -轉運蛋白基因mxirt1的490bp的片段，然後根據測序結果設計引物，通過race法從該cdna文庫中克隆了mxirt1基因的cdna全長。 -
By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。 -
The secondary structure of this protein speculated had a typically conserved domain of cytochrome p450 cysteine heme - iron ligand signature ( fnagprlcig ) and a hydrophobic domain at n - terminal of amino acid
（ 6 ） c鉀86mf同源基因片段在13個物種中的序列分析表明:它們的核昔酸差異為1 -
Comparison of conserved domains ( core - 2 / i - branching and wsc domain ) of oxt with mammalian homologues showed that both of domains are conserved throughout the invertebrate and vertebrate
將ox亡蛋白的保守結構域和哺乳動物的同源基因的保守結構域進行比較，發現這些保守區域在所比較的脊椎動物和無脊椎動物中都十分保守。 -
Blast in genbank indicated that it is novel gene named par - 1. the characteristics of the protein coded by this gene were analyzed by bioinformatics. the result showed that this protein was instable, p1 9. 65, located in bacterial inner membrane, and composed of alpha helix, extrended strand and random coil. there was a srong transmembrance domain and contained some conserved sites such as protein dinase c phosphorylation
通過網際網路數據庫及生物信息學分析工具進行初步分析表明：該基因編碼的蛋白是一種等電點為9 . 65的不穩定蛋白，定位於細胞內膜；由螺旋、延伸帶、和隨機捲曲三種形式組成，具有蛋白激酶c磷酸化位點等多個位點，並具有一個強跨膜疏水區。
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