cosmids 中文意思是什麼
cosmids
解釋
黏性質粒-
16 replacement plasmids for two different regions were obtained, but have n ' t been introduced into 10 - 22 successfully. 42 cosmids in this region were introduced into a heterogeneous host zx64, one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton
將300kb區域的柯斯質粒進行異源表達，發現有一文庫質粒5h4的轉化子在硫鏈絲菌素存在時表現出對棉花枯萎病菌微弱的抑制活性；無硫鏈絲菌素存在時，則檢測不到。 -
Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis. so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library. cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段為探針釣出了位於該區域的文庫克隆，並利用指紋印跡和雜交技術將這些文庫克隆排列起來，進而以位於該區域的不同位置的片段做臂構建了用於轉化和接合轉移用的基因置換質粒，並試圖通過基因置換將該區域置換下來，但尚未得到最終結果。 -
The work on physical mapping of the chromosome of s. nanchangensis ns3226 was initiated. nearly a full set of chromosomal asei - bamhi fragments of s. nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library. thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications
此外，還開展了南昌鏈黴菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈黴菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈黴菌ns3226的基因文庫中釣到164個陽性克隆，並從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。
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