cryptorchidism 中文意思是什麼
cryptorchidism
解釋
隱睪病-
Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。 -
In previous study, janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization, and registered in genbank
在前期的研究工作中，本室姜宏等通過建立小鼠隱睪模型，運用抑制消減雜交法（ suppressionsubtractivehybridization ， ssh ）克隆出24個小鼠睪丸生精細胞凋亡相關基因的est ，並在genbank中登錄。 -
A single dose of des caused a significant decrease in l - pgds expression, whereas testosterone propionate supplementation could restore l - pgds expression in the eds - treated rats. after unilateral cryptorchidism, l - pgds expression in rat abdominal testis and epididymis was significantly reduced as a time - dependent fashion from 5 days after operation
Eds處理后， l - pgdsmrna和蛋白在大鼠睪丸和附睪各段上皮中的表達明顯降低，而睪酮處理可部分恢復l - pgds在睪丸和附睪的表達。
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