cytometry 中文意思是什麼

cytometry 解釋
細胞計量術,細胞計數
  1. Application of flow cytometry in sperm chromatin structure assay

    流式細胞術在精子染色質結構分析中的應用
  2. In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test

    在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然後經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,並以伊紅y排斥試驗測定細胞的存活率。
  3. Using flow cytometry to detect peripheral blood eosinophils and their related molecules

    流式細胞術在外周血嗜酸性粒細胞及其相關分子檢測中的應用
  4. Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly. 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so

    3n ) ,將待測eaggec菌株的培養上清加入該報告菌株培養物中,用流式細胞術facs方法檢測報告菌株熒光強度的變化情況,間接反映ybt的表達與否。
  5. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型,運用組織化學、免疫熒光組織(細胞)化學、流式細胞儀分選方法( facs )以及分子生物學手段,研究了小鼠乳腺的發育規律:小鼠乳腺組織中類乳腺幹細胞:小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定;小鼠類乳腺幹細胞分化的潛能;小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明: 1
  6. The value of diploid peak before gi was larger in the higher concentration and longer time of arsenic trioxide on floe cytometry

    從凋亡細胞中提取dna片段在2瓊脂糖電泳,顯現典型凋亡dna片段142bp 、 174bp梯形帶。
  7. Immunophenotyping of peripheral blood leukemic cells by multi - parameter cytometry

    多參數流式細胞術對外周血白血病免疫分型的研究
  8. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母( schizosaccharomycespombe )為研究材料,通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ ( 2 + )的分佈及相對濃度,以及不同周期時相細胞中gfp - cam的熒光強度變化,並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  9. Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment

    用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用,並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平, mcf - 7 adr呈強陽性,而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % , mcf - 7細胞系細胞陽性率為1 . 8 % 。
  10. To facilitate supervision and management of food production and circulation, rapid methods for detection of foodborne pathgens, such as improvement and automatization of conventional methods, bioluminescent method, cytometry, impedancemetry and immunological methods were reviewed

    為方便對食品生產和流通的整個過程進行衛生監督和管理,就常規方法的改進和自動化、生物發光方法、細胞計數方法、阻抗測定方法和免疫學方法等食品中微生物的快速檢測方法進行了綜述。
  11. Surface markers on dcs were then analyzed by flow cytometry and the proliferation of t cells was detected by mtt colorimetry. resoults : peripheral blood monocytes from patients of carcinoma treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml for 7 days could observe dcs with typical morphology. simultaneously there was a decrease in cd 14 expression and increase in hla - dr, cd40, cd83 and cd86 on dcs

    結果,癌癥患者外周血中的單核細胞在rhgm - csf1000u ml + il - 4500u ml的條件下培養一周,就可看到典型的樹突狀細胞形態,其表面cd14分子表達減少, hla - dr 、 cd54 、 cd40 、 cd83及cd86分子的表達明顯增高,且具有明顯刺激t細胞增殖的能力,成功地完成了外周血單核細胞來源的dc的培養。
  12. The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased

    結合以上創建的方法和免疫熒光流式細胞術,用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養以模擬體內微環境,然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究,結果發現用腫瘤上清培養的pbmc細胞數量下降明顯,但同時對其有激活作用,且呈劑量依賴性;細胞數的下降主要是由細胞壞死和凋亡引起的,腫瘤上清對細胞周期沒有阻斷作用,反而略有促進作用; t細胞亞群比例增加,並促進表達th1 、 th2細胞因子。
  13. At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage

    在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化,結果表明,分裂期細胞總體熒光強度強于間期細胞;而對同一細胞內熒光強度的分析說明,間期細胞的熒光主要分佈於胞質中,細胞核內則分佈較少;而正在進行有絲分裂的細胞內熒光主要集中於赤道板處;剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。
  14. Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

    具體方法為: ( 1 )照射后12h ,收集nih3t3細胞,用流式細胞儀檢測各組細胞的細胞周期, pcr - sscp檢測抑癌基因p16的變化; ( 2 ) nih3t3細胞照射后立即收集細胞和細胞上清,用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化; ( 3 ) western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 ( mmp - 2 )的表達變化。
  15. Study on action of flow cytometry on apoptotic cell

    流式細胞儀對凋亡細胞作用的研究
  16. ( 4 ) cell cycle distribution by flow cytometry

    ( 4 )流式細胞儀檢測細胞周期分佈。
  17. Determination of trace silver by flow cytometry - scattering spectrometry

    流式細胞術散射光譜法測定痕量銀的研究
  18. Analysis of the effect of strong sound wave on plant cells cycles using flow cytometry

    流式細胞術分析強聲波對植物細胞周期的影響
  19. Pictured here is the flow cytometry pattern of a breast carcinoma

    圖示:乳腺癌流式細胞儀模式圖。
  20. Flow cytometry analysis of reticulated platelets : primal evaluation of clinical blood disorders

    流式細胞儀計數網織血小板及初步臨床應用
分享友人