d gene 中文意思是什麼

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Mccormack d g. calcitonin gene - related peptide vasodilation of human pulmonary vessels. j appl physiol, 1989, 67 : 1265

尚雲曉，袁壯，趙淑琴，等.哮喘患兒血漿降鈣素基因相關肽與內皮素水平相關研究.中國實用兒科雜志， 1996 ， 11 （ 3 ） : 146

Dec. 15, 2002, 30 : 5529 - 5538. 12 dixon d a, kaplan c d, mcintyre t m et al. post - transcriptional control of cyclooxygenase - 2 gene expression

隨著技術的不斷進步，我們相信結合基因轉錄產物量和mrna降解率可以更好地描述轉錄調節。

Association between vitamin d receptor gene polymorphism and multiple sclerosis

受體基因多態性與多發性硬化的相關研究

The individuals of rhd - positive phenotype with intact exons carried generally insert fragments and boxl box2 and box3 and this proved that inserts or rh box could n ' t affect the express of rh d gene. in 2 of the 5 wei nationality pedigrees whose proband were rh d - negative, rhc / e phenotype of all the rh negative individuals was ccee. rhd exon 4nsert and rh box did not be found in all individuals

在7個先證者為rhd陰性的漢族家系中，大部分成員均出現插入片段和rhbox ，且在遺傳上符合孟德爾遺傳定律， d外顯子完整且表型為rhd陽性的家系個體成員廣泛帶有插入片段和box1 、 box2或box3 ，插入片段或rhbox並未影響d基因的表達。

To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。

5. after induced with iptg, jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds, cultivate these e. coli with light or without light. the survival rate proved the gene of d. salina ( 6 - 4 ) photolyase has photoreactivation

5 .工ptg誘導過的轉化菌，經過30秒的紫外光照射后，分別進行光培養或暗培養，計算存活率，從而證明了及sa了z 』 na的（ 6一4 ）光裂合酶在大腸桿菌中可進行光修復活性作用。

By the technology of gene cloning, bioconversion of d - amino acids with engineered cells containing d - hydantoinase and d - carbamoylase would be expected to overcome the drawbacks presented by using the original strains described above. according to the reported amino acid sequence of d - hydantoinases, two primers were designed and synthesized

本文根據文獻報道的海因酶基因序列及大腸桿菌對密碼子的偏愛性分別設計了正向及反向引物，以基因組dna為模板，利用pcr技術擴增得到菌株pseudomonasputidayz - 26和sinorhizobiummorelensess - ori的d -海因酶基因。

In escherichia coli, arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway. here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog, and the effects of n - terminus on feedback inhibition and its quaternary structure, and the importance of the structural " d2 " symmetry to allosteric inhibition

本博士論文工作以大腸桿菌k - 12來源的arog為研究對象，通過定點突變、反饋抑制實驗和酶學動力學參數的測定，深入地研究了arog的反饋抑制位點的特性，並對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用，以及蛋白質結構的「 d2 」對稱性對酶功能的重要性等進行了具體的研究。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Analyzing gene organization, these two senv - d chinese isolates were analogous to that of the reported senv - d ( ax025730 ) isolate, two senv - h chinese isolates were a little different from the reported senv - h ( ax025838 ) isolate

分析其基因組結構及基因排列情況，兩個senv - d亞型分離株與已發表的對senv - d （ ax025730 ）株分析的結果相吻合。

In the presented study, using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v, the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated. we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression

本文以穩定轉染gnt - v正義或反義cdna質粒的h7721人肝癌細胞為材料，從下列四個方面對gnt - v影響信號轉導及其機制以及引起基因表達的變化進行了研究，企圖闡明糖基轉移酶轉染是怎樣調節細胞信號轉導的

Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran

3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ，並利用克隆的16srrna鄭州大學2003年博士學位論文

By using inverted microscope, it was observed that dunaliella salina of different growth stages after the high osmotic shocks can live in the medium with nacl concentration between 0. 1m and 5. 0m, but its growth status and period showed differently. the optimal concentration for the growth of dunaliella salina was 0. 5 - 1. 5m, and this organism could stand a variety range of osmotic shock. enolase gene, the anti - adversity gene of d. salina, was cloned by modified degenerate pcr technique

通過倒置顯微鏡觀察生長在不同鹽濃度，不同生長時期，以及經不同滲透壓震動的鹽藻，四川大學博士學位論文發現其在o . im一5 . omnaci培養基中均能正常生長，但其生活狀態及生長周期有所不同，其最適生長naci濃度為0 . 5一1 . 5m ，還能適應各種高滲及低滲震動。

The future characterization and genetic analysis for candidate mutant were carried out and find that some candidate mutant ( such as roi30 doil - 1 doi0311131 ) have good phenotype by drought h2o2 aba - stressed treatment. at the same time we also observe the development of candidate mutant at different growth stages carefully. many modal difference between mutant an d wild type at the same period were found, such as more rosette layering fatty and big in leaves, advancment or delay for the flower period, rosettes living in the main stem, shorten in figure, the amount of seed little, sterilization etc. these physiological and modal changes may reflect with maladjustment in expressions of some gene and confusion on their inner control, . we will futher study concrete and detailed function mechanism

我們對這些擬南芥侯選突變體進行進一步的鑒定和遺傳學分析，發現ro口口、 doil 、 doi口jlll3i等潛在突變株對aba 、過氧化氫及早脅迫有明顯表型，同時對潛在突變體的生長發育進行了詳細的觀察，發現多數潛在突變株與同條件下野生型比出現了許多明顯的形態改變，如:蓮座基葉增多、分層、肥大，花期提前或延遲，主莖生輪座，株型矮化，產籽量少，不育，敗育等，這些生理和形態上的差異很可能反映了它們內部某些基因的表達受到了影響、代謝調控發生了紊亂，具體和詳細的作用機制還需要進一步的研究。

Studies on the introduction of the cecropin d gene into eucalyptus urophylla to breed the resistant varieties to pseudomonas solanacearum

基因轉化桉樹培育抗青枯病株系的研究

Biological rule of inserts and rh box needs farther research. our research showed that gene sequences of the oriental were different from that of caucasian. designing primers according to rh dna sequence derived from caucasian could not discover the influence factors of rh d gene expression and inheritance characteristics

筆者認為，東方人種有著不同於高加索人的rh血型基因u ，文獻公布的rhdna序列來源於高加索人種，據其設計引物進行的研究，不能完全揭示中國人d基因表達的影響因素及遺傳學特徵。

The polymorphism of rh d gene structure of han nationality of chinese was significantly different from caucasian and wei nationality characteristic

中國漢族人群在rhd基因結構多態性上與高加索人種顯著不同，而維族人群則另具特徵。

( 2 ) gene structure of the rh d - negative in chinese have polymorphism, especially in the individuals with c antigen, which accorded with the oriental character. d gene exon of chinese polymorphism expressed for intact, partial deletion and total deletion, and probably existed specific d ( nf ) ce haplotype

中國人rhd基因結構具有多態性，特別是帶有c的個體，符合東方人具有完全缺失、部分缺失和不缺失三種情況，並可能存在特殊的d （ nf ） ce單體型。

The relation of insert and segment and rh box with the expression of rh d gene was uncertain in our research. this inherited characteristic was different from caucasian

本組樣本的漢族和維族家系，都不能確定插入片段和rhbox與d基因表達的關系，這一遺傳學特徵不同於高加索人種。