deacetylase 中文意思是什麼
deacetylase
解釋
去乙醯酶-
High - property chitosan can be produced by chitin deacetylase
而採用甲殼素脫乙酰酶能制備出性能獨特的殼聚糖。 -
In this paper, advances in the application and research of chitin deacetylase in preparation of chitosan are summarized
本文就甲殼素脫乙酰酶在殼聚糖制備中的應用及研究進展作一綜述。 -
At mean time, we have studied on the characteristic of proteinase and make sure the suitable condition and influencing factor of deacetylase function. usin g the technology of immobiolized cell, the utlization of deacetylase have been increased deeply and lower production cost. the half deline period is more than 20 days and the production of l - methionine is above 75 %
採用固定化細胞技術，使酶的利用率得以大幅度提高，固定化細胞酶活的半衰期達20天以上，可連續拆分使用生產24天， l - met產率可達75以上。 -
5, histone acetyltransferase gene ygcns and histone deacetylase gene yrpd3 were cloned and expressed, which has laid down a foundation for studying chromosome remodeling in vitro
5 ，我們克隆並純化了酵母中的組蛋白乙酞基轉移酶基因gcns和去乙酞化酶基因rpd3 。 -
6, we used gcn5 and rpd3 genes as probes to detect the homologous sequences in drosophila melanogaster by fluorescence in situ hybridization ( fish ). this work has provided useful information for the localization and cloning of related histone acetyltransferase and histone deacetylase genes in drosophila melanogaster
6 ，利用己獲得的酵母gcns和rpd3基因為探針，對果蠅多線染色體進行原位雜交實驗，試圖找出與gcns和rpd3基因同源的基因片段。為今後克隆和分離果蠅中與乙酞化和去乙酚化相關的基因奠定基礎。 -
By treating the cells in s, g2 phase and prophase with histone deacetylase inhibitor tsa, and through the application of microscopic observation and western - blotting, we demonstrated that histone acetylation modification played important roles in the cell cycle regulation in physarum polycephalum, affecting the normal crossover of the checkpoints of s / g2, g2 / m and mitosis exit
這些炎白的表達水平具有細胞周期依賴性，隨著細胞周期的進行而發生變化。 tsa處理引起的多頭絨泡菌s期、 gz期和前期細胞內組蛋白h3乙酚化水平的提高，改變了細胞內類cyclinbi蛋白、類。 -
Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。 -
1, the histone deacetylase inhibitors were used to feed the larve of the fly, and then the polytene chromosomes were observed under the microscope. the results indicated that the histone deacetylase inhibitors had effects on the morphology of the polytene chromosomes. it is suggested that the acetytion has effects not only on the molecular interaction but also on the structure of the chromosomes
得到的主要結果和結論如下： 1 ，通過用去乙酰化酶抑制劑處理果蠅幼蟲，觀察果蠅多線染色體在熱休克基因處的形態變化，發現去乙酰化酶抑制劑介導的乙酰化水平的升高可引起染色體結構的顯著變化，推測乙酰化修飾不僅影響分子間的相互作用而且還可以影響染色體的高級結構。 -
4, compared the influence of two histone deacetylase inhibitors on the hsp70 gene transcription, after changing the time and the concentration of the inhibitors, we found the mechanism of the two histone deaceylase inhibitors is different
4 ，通過比較改變餵食時間和餵食濃度后兩種去乙酞化酶抑制劑與熱休克基因表達水平之間的關系，我們發現tsa與丁酸鹽抑制去乙酞化酶的作用機制可能是不同的。 -
2, the hsp gene transcription was quantitatively determined by rt - pcr. based on this result, it is concluded that the change of acetylation level at the loci of hsp, mediated by histone deacetylase inhibitors, exerts important functions in hsp gene transcription. 3, after immunolabeling with anti acelated - lysine monoclonal antibody on the polytene chromosomes of heat shocked flies, fluorescence signals were detected at the hsp loci
2 ，利用rt - pcr技術，對去乙酰化酶抑制劑處理后的果蠅的熱休克基因的表達水平進行檢測，結果表明經組蛋白去乙酰化酶抑制劑處理后的果蠅幼蟲，其熱休克基因的表達高於基礎水平，也就是說去乙酰化酶抑制劑誘導了熱休克基因的表達。
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