delete segment function 中文意思是什麼
delete segment function
解釋
刪除段功能-
There is no characteristic in the amino acid sequence 63 - 152 and it is the piece that we want to delete to identify the function of the segment. ie180 gene mutants deleted the 64 - 151 amino acid was amplified by muti - pcr and were cloned by pmdist - vector. the clone plasmids were named pjmp1p3p2. the segment corresponding to the sequence of 1 - 1079 amino acid of the genbank sequence amplified by pcr, its clone plasmids was named pjmp1p2. the segment corresponding to the sequence of 454 - 1079 of genbank sequence amplified by pcr and its clone plasmids is named pjmp6p2 - three clone plasmids and pcdna3 were digested by restriction enzyme bamhi and hind, the gene segment of p1p2, p1p3p2, p6p2 were recycled
本試驗應用dnamis及prosis軟體分別對genbank中登記號為no352564的ie180序列進行了蛋白質序列分析,結果表明其1 - 34段氨基酸序列具有典型helix - turn - helix特徵序列,並且富含酸性氨基酸d及e ,是典型的dna識別序列;富含絲氨酸序列的152 - 409氨基酸序列是一個與激活有關的、潛在的磷酸化位點; 454 - 696氨基酸區域是dna結合域; 64 - 151氨基酸片段沒有明顯的序列特徵;從中可看出ie180蛋白的1 - 1080氨基酸段具有典型的轉錄激活因子結構特徵。
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