demecolcine 中文意思是什麼
demecolcine
解釋
地美可辛 秋水仙胺-
Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。 -
After 15h of maturation. 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended. secondly, strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium
第一次減數分裂期小鼠卵母細胞經化學去核后，去除透明帶，並胎兒成纖維細胞粘合、電融合併srcl2激活，培養于微孔中。
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