dhfr 中文意思是什麼

dhfr 解釋
二氫葉酸還原酶
  1. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
  2. In this study, we first constructed a vector containing human elongation factor la subunit promoter ( pef - 1 ) for transcription of genes of interests, and mouse dihydrofolate reductase ( dhfr ) gene under control of sv40 promoter ( psv4o ) for clonal selection and amplification. the vector was named ped5

    本研究首先構建了含有人延伸因子1亞基啟動子（ p _ （ ef - 1 ） ）和小鼠二氫葉酸還原酶（ dhfr ）加壓擴增基因的表達載體ped5 ，用於外源基因的常規表達。
  3. Furthermore, the egfp - eo gene was also cloned into pci - dhfr, a cho ( dhfr " ) cell expression vector. following the transfection and mtx selection, some cho cells presented green fluoresence, which will establish a good foundation for production of soluble eo glycoprotein and investigating its biological functions

    將p2種子液以moi5 - 10接種指數期sf9細胞，三天後呈現出最強的熒光。我們還將egfp - eo融合基因插入哺乳動物細胞表達載體pci - dhfr中，構建重組質粒pci - dhfr - egfpeo 。
  4. 2. to construct the prokaryotic expression vector and the control vector. after sequenced, the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr

    將自puc18 h d - 3回收的目的片段連同表達運載蛋白dhfr的基因片斷一起連接于原核高效表達載體pqe - 80l ，經酶切鑒定，得到重組原核表達載體pqe - 80l dhfr h d - 3 。
  5. The recombinant vector pqe - 80l / dhfr / hpd - 3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy. 3

    同時還將表達運載蛋白dhfr的基因單獨連接于表達載體pqe - 80l ，得到對照質粒pqe - 80l dhfr 。
  6. ( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr. an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii

    Sds一隊ge分析可見重組原核表達載體pqe一80l / dhf側hpd一3表達的目的蛋白帶和對照質粒pqe一sol / dhfr表達的蛋白帶，大小分別約為31kd和26kd 。
  7. An about 550 bps and an about 700 bps fragment could be seen after the vector pqe80l / dhfr / hpd - 3 was digested by bamh i + kpn i and by bamh i + hindiii r

    經w亡sternblot證實，在大小分別約為3ikd和26kd的位置有兩條清楚的蛋白帶。
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