dilution assay 中文意思是什麼

dilution assay 解釋
稱稀釋測定
  • dilution : n. 1. 沖淡；稀釋。2. 稀度，淡度。3. 稀釋物。4. 削弱，削減。5. （把技術性操作分成若干加工過程而用生手替換熟手的）勞動力的削減 （＝ dilution of labour）。
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. From the information available for the preparation to be assayed ( the “ unknown ” ), assign to it an assumed potency per unit weight or volume, and on this assumption prepare on the day of the assay a stock solution and test dilution as specified for each antibiotic but with the same final diluent as used for the usp reference standard. the assay with 5levels of the standard requires only one level of the unknown at a concentration assumed equal to the median level of the standard

    根據有效的信息制備試驗溶液，指定一個假定的每單位重量的效價或者體積，然後在假設的基礎制備當天儲備液試驗和稀釋溶液作為每一種抗生素指定的效價，但是當使用相同usp參考標準品的最終稀釋液時，試驗應使用平行5的標準要求僅有一份未知的做為濃度等同於中部的標準。
  2. The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d

    為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。
  3. Diluting virus with 0. 85 % nacl into concentration of 10 - 1 - 108, the normal mice were challenged by intranasal inoculation of a range of 200ul virus dilution to assay its ld50

    以10一儀10一，八個稀釋度的病毒鼻腔接種小鼠， 0 . lml /只，測定其小鼠半數致死量（ lds 。
  4. Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution

    該純化cpe對小白鼠的半數致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。
  5. Methods : sandwich elisa assay was used, w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody. the second antibody - hrp conjugate was added for coloration. standard curve was obtained by shla - i standard reagent in serial dilution. the amount of shla - i in the samples was determined : 1

    方法：以w6 32包被酶標板，捕捉樣品中可溶性hla ，加入一抗2m抗體，再加酶標二抗及底物顯色。根據可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線： 1
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