direct sequencing 中文意思是什麼

direct sequencing 解釋
直接測序法
  • direct : adj 1 筆直的，一直線的；正面的。2 直接的。3 直截了當的，直率的，明白的。4 直系的，正統的。5 【語...
  • sequencing : 測序，序列測定
  1. A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing

    提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。
  2. By analyzing the wing phenotype, cuticle phenotype of germline clone embryo, deficiency kit mapping, direct sequencing and searching the genome sequence database, three genes : sll, oxt and pygo were identified ( each gene corresponding to one group of mutant alleles )

    並通過綜合分析翅的形態，生殖系克隆胚胎的表皮形態，以及缺失作圖，直接測序和搜索基因組數據庫，鑒定出了三個基因，這三個基因分別對應于該三類突變體等位基因。
  3. Compared with the results of dma direct sequencing, pcr - sscp has the same results as that except for failing to check out the rpob gene mutation of a rifampin - resistant isolate. it proves that pcr - sscp is a rapid and sensitive mutation testing method

    與dna測序結果相比較， pcr - sscp除一個耐藥株的rpob基因突變沒有檢出外，其它檢測結果均與dna測序結果相一致，這進一步說明pcr - sscp是一種快速、靈敏的突變檢測技術。
  4. Used long pcr primers as sequencing primer, the two sides of sequence of the long pcr product were separately sequenced one reaction by the direct sequencing method of pcr products, and the sequencing sequence were carried on constrasted analysis with sequence of 168 strain in genebank

    一2片段克隆質粒為模板，採用skippcr方法，得到了約4 . 3kb產物，以ta克隆方式，插入pgem一t載體中。將經鑒定的陽性克隆質粒進行測序，結果表明: biob與biol基因間的5lbp的終止子序列已被刪除。
  5. Then, to get more knowledge about the rpob gene mutations and provide some scientific information to study the molecule mechanism of resistance to rifampin in mycobacterium tuberculosis, the rpob gene sequence of mycobacterium tuberculosis clinical isolates from shannxi was analysed by direct sequencing of pcr products

    隨后通過直接測序法對陜西區域內結核分枝桿菌臨床分離株rpob基因pcr擴增產物進行序列分析，了解該區域rpob基因突變情況，為研究結核分枝桿菌耐利福平的分子機制提供參考。
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