directed mutagenesis 中文意思是什麼

directed mutagenesis 解釋
定向突變
  • directed : adj. 1. 有指導的；定向的。2. 【數學】標出（數、角、線段的）正負的。
  • mutagenesis : n. 突變。
  1. Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases

    研究人員已經利用蛋白質工程和定點突變技術來改變各種醇腈酶的活性位點和底物特異性等。
  2. The principles, operation and applications of site - directed mutagenesis, gene fusion technology, and post - translational modification methods were introduced emphatically

    著重闡述了基因定點突變技術、基因融合技術和翻譯修飾技術等新興定點固定化技術的原理、特點和操作。
  3. The experimental procedure that begins with a cloned segment of dna, or a protein sequence, and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function

    在已知dna克隆片段或蛋白質序列上引導程序性的變異（通過直接誘變）並返回到基因組，來研究基因和蛋白質功能的實驗方法。
  4. We obtained the full length gene of hbfgf coding sequence with pcr, and adjusted the g + c content according the software dnasisv2. 5, and replaced the cys78 and cys96 with serines by site - directed mutagenesis. 2. sequence result suggested one of the recombinant is correctly synthezied and cloned

    用pcr方法合成了hbfgf編碼區全長，其中前20個氨基酸的g + c含量按暨南大學碩士學位論文：大腸桿菌表達重組hbfgf結構和功能優化摘要照計算機軟體計算的結果進行了調整，第78和96位上的半瞇氨酸被突變為絲氨酸。
  5. Direct damage on supf trna gene can be neglected because half - life of mnng is 1. 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours. therefore the mutagenesis is not lesion directed

    20m的mnng經洗滌后的極微量殘留經過10 ? 20多個半衰期的降解后，己不足攻擊轉人的qdna分子，因此這種突變顯然是發生在mnng直接攻擊部位以外的正常堿基上。
  6. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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