divergent sequences 中文意思是什麼
-
In contrast, sequences from two strains gymnodinium are divergent ( the sequence similarities of the small subunit ( ssu ) rdna, the its1, the 5. 8s rdna, the its2 and the large subunit ( lsu ) rdna are 76. 5 %, 25. 4 %, 54. 2 %, 23. 2 %, 56. 5 %, respectively ), though their phylogenetic positions are relatively close
,編號為gyn - 15 ) 、一株米氏裸甲藻( gymnodiniummikimotoi ) )雖然進化距離接近,但它們的rdna序列相似性卻很低,全長序列的相似性僅為54 . 1 。 -
On the other hand, studies on the quantitatively rapid detection using the divergent sequences of rdna are one of the most common focuses on phytoplanktonic ecology
同時,以rdna序列多樣性為基礎建立浮游植物的定量檢測方法,是目前浮游植物生態學研究的熱點之一。 -
The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced
首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域,共設計出7對適合rfq - pcr的引物(依次命名為primer1 ( f r ) primer7 ( f r ) ) ,並用常規pcr實驗初步證明其中有4對引物( primer1 ( f r ) 、 primer2 ( f r ) 、 primer5 ( f r ) 、 primer6 ( f r ) )可作為中肋骨條藻特異性引物的候選者;然後測定了以primer6 ( f r )為引物的pcr產物的序列,序列分析表明,中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大,從中可設計出滿足rfq - pcr需要的taqman探針(命名為taqman6 ) ;進一步的核酸雜交實驗表明, taqman6隻與中肋骨條藻的pcr產物雜交,不與其他藻的pcr產物雜交。
分享友人