dna amplification 中文意思是什麼

dna amplification 解釋
dna 擴增
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • amplification : n. 1. 擴大；擴充。2. 【電學】增幅，放大（率）。3. （聲明等的）補充材料。
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. With the improved method of ctab, the genomic dna extracted form plum and other species close to p. mume in genetic relationship could be directly used in pcr amplification, with good results obtained

    選用改良ctab法提取的梅及其近緣種的基因組dna可直接用於pcr擴增分析，且效果良好。
  3. The results showed that dna of tannage could be extracted by this method and used as samples in conservation genetics. it is a good idea for molecular identification of leather things and amplification or sequencing of genes

    結果證明，運用這種改進的提取方法從鞣製皮革中提取的總dna適合保護遺傳學研究的需要，可用於部分序列的擴增和測序研究及揚子鱷皮質用品等的分子鑒定。
  4. Sixties primers were used for dna amplification and a total of 237 amplification products range from 450bp to 2500bp were generated. a similarity matrix for all pairwise comparisons was calculated using nei and li ' s formula and then transformed to distance matrix. dendrograms were constructed by applying unweighted pair - group arithmetic average ( upgma ) and neighboring - jointing cluster analyses using the phylip software

    在第二部分，應用改進的ctab法提取了石蓴屬和滸苔屬各3個種及作為對照的剛毛藻的基因組dna ， 60個引物被用於擴增，共獲得237個片段大小在450 - 2500bp之間的擴增片段，依據nei和li （ 1979 ）的公式計算出樣品成對比較的相似性距陣並換算成遺傳距離，應用phylip軟體包，按照upgma法和n - j法分別構建聚類圖。
  5. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  6. The association of larval and adult stages of ecologically important caddisfly ( insecta : trichoptera ) using mitochondrial dna sequences larval and adult amplification fragments were cloned and sequenced. dna sequences were aligned using clustal x software ( gene codes corporation )

    線粒休dna一coi 、 u基因序列在毛翅目成幼蟲聯系中的應用研究擴增並測定了4種鱗石蛾的成、幼蟲的mtdnacoi 、 coh及trna一leu基因序歹『 j 。
  7. In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector

    先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。
  8. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  9. In chapter three, genomic dna were extracted from four individuals from sinotubimorpha porracea and grateloupia filicina, respectively and from g. livida as a control. sixties primers were used for dna amplification and a total of 120 amplification products range from 250bp to 2500bp were generated. distance matrix and dendrograms were obtained as described in last paragraph

    在第三部分，應用ctab法提取了管形藻、蜈蚣藻各4個個體及作為對照的舌狀蜈蚣藻的基因組dna ， 60個引物的rapd分析獲得120個片段大小在250 - 2500bp之間的擴增片段，遺傳距離和聚類分析同前。
  10. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。
  11. The results showed about 490bp dna fragments were amplified. because the amplified products is specific to the p - subclass of the proteobacteria, the amplification of the amoa gene may be a powerful molecular tools for detecting and analyzing ammonia - oxidizing communities in environment

    由於基於此引物的擴增對proteobacteria -亞科氨氧化細菌具有特異性，所以amoa基因片段的特異擴增為我們檢測和鑒定環境樣品中氨氧化細菌的種群提供了一個有效的工具。
  12. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  13. 3. its shown from the results of dna digestion, hydantoinase gene amplification, rapd, eric - pcr etc, that the electrophoretic patterns of the products are obviously different form the original one

    分別提取的基因組后進行酶切分析、海因酶基因的擴增、 rapd 、 eric - pcr等檢測，兩者均呈現不同的電泳圖譜。
  14. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  15. By sun, bo ( major : marine biology ) directed by professor zhang, guofan and dr. liu, xiao population genetics of four argopecten irradians cultured populations was analyzed by using random amplification polymorphism dna ( rapd ) techniques. and partial dna sequences of the internal transcribed spacers ( its ) from four representative samples ( one sample represent for each population ) were amplified by pcr and sequenced in order to stress the phylogenetic relationships of the four populations. besides, by rapd technique, two argopecten irradians families were studied to examine whether the hybridization experiments were successful and which family was more suitable to be materials in future genetic linkage map construction

    本文以海灣扇貝4個養殖群體為研究對象，採用隨機引物多態性dna （ rapd ）技術進行了群體遺傳學研究，並嘗試使用4個群體中代表個體的內轉錄間隔區（ its ）序列比對分析結果作為參考；另外，本文還運用rapd技術對人工異體交配的2個海灣扇貝家系的雜交成功率進行了分析，均獲得成功證實，並評估了兩個家系構建遺傳連鎖圖譜的潛力。
  16. After one - step reverse transcription of the rna virus genomes, the viruses " specific dna fragments were amplified by nested three loci pcr, which the amplification system was optimized by uniform design

    選擇合適的核酸抽提方法，一步法抽提hbv 、 hcv及hiv 1的病毒核酸並一步法逆轉錄病毒的rna ，獲得病毒的dna 。
  17. The aflp ( amplified fragment length polymorphism ) technique is based on the selective pcr amplification of restriction fragments from genomic dna digested by restriction enzymes completely

    擴增片段長度多態性技術（ aflp ）是基於選擇性擴增完全酶切消化后的基因組dna片段。
  18. Pcr - based analysis method was carried out in the following study. dna amplification by pcr in cgg repeats were performed on 154 people as normal control ( 73 males and 81 females ) and 23 members from four x - linked mental retardation ( xlmr ) families. after electrophoresed on a 6 % - denaturing polyacrylamide gel ( acr : bis = 19 : l ), genotypes of the objects were determined by analyzing pcr products

    本研究採用較以往更為簡便有效的pcr技術方法，對隨機抽取的154人的正常群體（男性73人、女性sl人）及來自四個智力低下家系的23名成員進行了fmr基因cgg重復序列的擴增，利用變性聚丙烯酸胺凝膠電泳技術對不同等位基因類型進行分離和統計。
  19. Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion, and high - level dna amplification of those sequences flanking the deletion

    許多鏈黴菌表現出高度的遺傳不穩定性，通常為大片段缺失或基因組重排，以及缺失區域兩側序列的高水平dna擴增。
  20. Polymerase chain reaction is a rapidly developing and widely used dna amplification technique, which is widely applied in life science and other related fields

    聚合酶鏈式反應（ polymerasechainreaction ，簡稱pcr ）技術是發展很快、應用很廣的體外擴增基因片斷技術，在生命科學研究及諸多相關領域已經得到了廣泛應用。
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