dna blotting 中文意思是什麼
dna blotting
解釋
dna印跡（法）-
This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting
本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )
用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3 -
705bp dna fragment of mxnrampl gene and full cdna of mxlrtl gene which were related to resist iron stress were cloned by using malus xiaojinensis cheng et jiang - the first iron - efficient genotype in the genus malus in the world as material. ( 1 ) using fragment of nramp gene from wheat and fe ( ll ) - transporter gene fragment of maize ( zmlrt ~ ) as probes, we analysed these genes by blotting hybridization technique in malus xiaojinensis cheng et jiang
本實驗以中國農業大學園藝植物研究所篩選到的一個蘋果鐵高效基因型? ?小金海棠（ malusxiaojinensischengetjiang ）為試材，分別克隆了小金海棠的抗缺鐵相關基因mxnramp1基因的752bp基因組dna片段和fe （ ） -轉運蛋白基因（ mxirt1 ）的cdna全長，為深入探討小金海棠抗缺鐵的分子機理奠定了基礎。 -
Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa
將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。 -
Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。 -
They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection
用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核，並移植入假孕受體鼠，產生的65隻n代小鼠中，經pcr和southern雜交檢測，表明轉入的基因在12隻小鼠體內有整合段。 -
Pcr analysis and southern blotting all confirmed that the ced - 9 gene has been integrated into the chromosomal dna of these transgenic plants
對所得到96株再生苗進行pcr檢測，結果表明，陽性苗比例為34 。 -
The dna and rna dot blotting revealed transcription of the polyprotein gene into mrna in the vero cell
比較了dna疫苗和兩種常用的弱毒苗（ b87 、 d78 ）的安全性和免疫效力。 -
Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80. 3 % of them were positive. the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2. 5 copy of t - dna
對抗性植株進行pcr擴增檢測，結果表明有80 . 3的抗性植株為陽性植株； southern雜交結果進一步證明了質粒的t - dna已經整合到水稻的基因組中，拷貝數為1 4個，平均每棵轉化植株有2 . 5個拷貝。
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