dna electrophoresis 中文意思是什麼

dna electrophoresis 解釋
dna電泳
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • electrophoresis : n. 電泳（法）。
  1. Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2. the migrating distances of dna of brain, lung, spleen and kidney cells of mice increased significantly, compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0. 2 % ), and lead could strengthen dna damage degree by so2 in nuclear dna of brain, kidney, spleen cells. damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead

    為了明確大氣顆粒物中的重要組分? ?鉛在二氧化硫所致dna損傷中的作用程度，利用單細胞凝膠電泳技術（ singlecellgelelectrophoresis ， scge ，或稱彗星實驗， cometassay ）研究了鉛與二氧化硫的聯合污染，結果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract鉛單獨及聯合染毒條件下，小鼠腦、肺、腎、脾細胞dna遷移距離均比對照顯著增加;鉛加劇了50 :對腦、腎、脾細胞核dna的損傷程度; 50 :對肺細胞核dna的損傷程度要比鉛的損傷大，小鼠肺細胞核dna遷移距離在50 :和醋酸鉛聯合作用組與醋酸鉛單獨作用組間有極顯著性差異（ p < 0 . 01 ） ，而與502單獨作用組間沒有顯著性差異。
  2. ( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis

    （ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。
  3. In this sense, ida plays dual effects, tridentate chelating to cu ( 2 ) and bridge between two cu ( 1 ) andcu ( 2 ). 3 metal complexes were selected as the appropriate for the study of cleavage plasmid pbr322dna by gel electrophoresis technique. the results showed ni and mn complexes could cleave effect - ively dna in the presence of h2o2 at physiological ph and temperature, whereas individual zn complex could cleave effectively dna

    通過電泳實驗研究了一系列金屬配合物與pbr322dna的作用，發現在tris - hcl緩沖溶液中，生理條件下，鎳、錳配合物在共反應物h _ 2o _ 2存在下能夠很好的斷裂dna ，而zn配合物單獨作用就能夠使dna由ccc型轉化為oc和linear型。
  4. The mechanisms of such treatment have been proposed as inhibition of proliferation and angiogenesis, as well as induction of differentiation and apoptosis, as has been tested by various in vivo and in vitro experiments. in our experiments, it has also been demonstrated that after the treatment of arsenic trioxide, the k562 cells has undergone major morphological changes, which included nuclear shrinkage, membrane bleb and scattered apoptotic bodies. dna gel electrophoresis also discovered that the typical " dna ladder " phenomena in the treatment group, while the control group showed the regular genomic banding

    我們在實驗中觀察到as _ 2o _ 3作用人紅白血病k562細胞后，細胞生長明顯變緩，部分細胞出現皺縮、染色質濃聚及胞膜起泡現象，部分細胞胞膜破裂，在其周圍有緻密的凋亡小體出現， dna電泳出現典型的凋亡「梯狀」帶，提示as _ 2o _ 3能有效抑制k562細胞生長，誘導k562細胞凋亡。
  5. The equipment here may be pipette, electrophoresis system capillary, transblot system, chest / oven molecule hybridization, water bath, dna sequencer, microplate - reader for elisa, microplate washer and so on

    本區所使用的儀器設備可能有加樣器、電泳儀（槽） 、電轉印儀、雜交爐或雜交箱、水浴箱、 dna測序儀、酶標儀和洗板機等。
  6. The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis

    本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。
  7. Apoptotic peak. agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a

    電泳出現階梯狀條帶流式細胞儀檢測出現典型的凋亡峰，提示
  8. In the second part, polyphasic taxonomy methods such as morphological method, physiological and biochemical method, dna g + cmol %, soluble protein electrophoresis and 18s rdna sequence analysis were used for systematics of all strains isolated and purified with the method above

    本論文第二部分採用形態學、生理生化特徵、 dnag + cmol 、可溶性蛋白電泳及18srdna序列分析等分類技術對所分離的16株甲真菌進行了系統的分類研究，從而初步確定了16株甲真菌的分類地位。
  9. In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans, we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining

    為了研究耐輻射球菌極強的輻射抗性與dna修復機理，我們應用雙向電泳結合銀染的方法考察了野生型菌株kd8301在射線照射前後細胞體內總蛋白組的變化情況。
  10. Location of the hbrp gene in the chromosome according to stanford g3 rh panel, a pair of primers are designed in the 3 " - utr region of the gene which is to be localized and human genome dna is amplified in advance whose products are examined by electrophoresis. then g3 panel is amplified and this process is repeated twice. the pcr result is sent to stanford human genome center ( shgc ) http : / / www - shgc

    Hbrp基因的染色體定位使用stanfordg3rhkadiationhybrid ）嵌板，在所要定位基因的3 』非翻譯區（ untranslatedregion ， utr ）設計引物，預先擴增人基因組dna ，電泳檢測產物大小，繼之擴增g3rhpanel ，重復兩次，將pcr結果輸人斯坦福人類基因組中心（ shgc ）的主頁（ http ： 、 shgc
  11. The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis. the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands. apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells

    4 .用電泳技術研究亞稀褶黑菇粗毒液誘導小白鼠肝腎細胞凋亡，小白鼠亞稀褶黑菇抽提液中毒后，肝腎細胞. dna經瓊脂糖凝膠電泳出現180一200bp整數倍的ona梯形帶， 19 . 09 / l一28 . 59 / l范圍內，亞稀褶黑菇提取液誘導肝腎細胞凋亡表現出時間和劑量依賴性
  12. ( 2 ) effects on mouse spleen of so2 challenge : we found significant apoptotic changes of mouse spleen through tem observation and dna electrophoresis analysis and flow cytometric analysis. we found condensed, marginating, half - moon like apoptotic lymphocytes both in white pulp and red pulp ; we found significant dna degradation with dna ladders from the dna electrophoresis analysis in the 168 mg / m3 so2 treated group ; we also found marked increase of apoptotic rate between 168 mg / m3 so2 treated group and control group from the flow cytometric analysis

    （ 2 ）二氧化硫吸入可引起小鼠脾臟細胞出現明顯的凋亡改變，紅髓區和白髓區淋巴細胞出現核固縮，染色質凝聚、邊集； dna凝膠電泳分析發現168mg m ~ 3二氧化硫染毒組出現典型的dna梯形條帶；流式細胞分析也發現高劑量染毒組的小鼠脾細胞凋亡率增加，並且與對照組相比有顯著性差異， p 0 . 05 。
  13. In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226

    將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。
  14. In order to understand the mechanism of mtx further and to investigate the genotoxic target organs, we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay. liver, spleen, bone marrow, thymus, kidney, testicle, stomach and peripheral lymphocytes of mice were isolated at lh, 3h, 6h, 12h, 24h after 5mg / kg mtx intraperitoneal injection

    為了進一步了解甲氨蝶呤（ mtx ）的作用機制，探測其作用的遺傳毒性靶器官，為應用mtx治療過程中的臨床監測和副作用防治提供理論依據，我們以小鼠為研究對象，用單細胞凝膠電泳技術檢測了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后對肝、脾、骨髓、胸腺、腎、睪丸、胃和外周血淋巴細胞的dna損傷作用及損傷程度與mtx劑量間的關系。
  15. Wear gloves because the electrophoresis plasticware could be contaminated with the carcinogen ethidiumbromide, that is used to visualize dna

    請穿戴手套！因為電泳用的塑膠製品可能被用來觀察dna的致癌物溴化乙錠所污染。
  16. The results were as following : 1. construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology, sweet protein thaumatin gene was cloned into plant expression vector pbii2i. recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis

    甜蛋白thaumatin基因植物表達質粒pbi _ （ 121 ） - th的構建與鑒定利用dna重組技術，將植物甜蛋白thaumatin基因克隆至植物表達載體pbi _ （ 121 ）中，通過酶切、電泳，鑒定thaumatin基因已成功構建到植物表達質粒pbi _ （ 121 ）中。
  17. In order to understand the epidemiological behaviors of this pathogen, we studied strains e. coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city, jiangsu province, by methods of pcr, antimicrobial susceptibility, biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal

    為了探索我國大腸桿菌o157 ： h7感染的流行病學特點，我們使用了聚合酶鏈反應、生化反應、抗生素敏感實驗、脈沖電場凝膠電泳等分子生物學方法，對我國1999 - 2000年徐州地區分離到的部分大腸桿菌o157 ： h7進行了分析。
  18. Dna ladder bands with a periodicity of about 200 bp were clearly seen in agarose gel electrophoresis pattern

    瓊脂糖凝膠電泳基因組dna可見到約以200bp為間隔的dna梯狀（ ladder ）條帶。
  19. Its genomic dna was partially digested by sauial. dna fragments from 4 to 16 kb were collected after electrophoresis and ligated with bamhi - digested puc18 to construct genomic library. the total number of recombinant plasmids is about 9000

    進一步在opua基因的上下游序列分別設計引物，從h . trueperi基因組dna中擴增出所預期大小的片段，測序驗證已獲得opua基因的全序列。
  20. Apoptosis was identified by dna electrophoresis and flow cytometry. the results showed the supernatant containing hsblys induced a does and concentration - dependant increase in apoptosis cells

    Dna瓊脂糖凝膠電泳和流式細胞儀結果顯示， hsblys表達上清可誘導k562細胞凋亡，且具有劑量依賴效應。
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