dna homology 中文意思是什麼

dna homology 解釋
dna同源性
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • homology : n. 1. 相應，符合；關系相同。2. 【化學】同系（現象）。3. 【生物學】同源。4. 【數學】透射；同調。
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant

    本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。
  3. Tsarg2 with 1 233 bp length was composed of 6 exons and spaned about 115 kb of genomic dna, the putative protein encoded by this gene was 305 amino acid with a theoretical mass of 34 751 and with no significant homology with any known protein in databases. a kind of nucleoprotein was the most impossible

    Tsarg2基因的cdna全長為1233bp ，包含6個外顯子，基因組跨越115kb ，編碼由305個氨基酸組成的、分子量為34751的蛋白質，與已知蛋白質無明顯同源性，其最大可能是一種核蛋白。
  4. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列，設計一對兼并引物， pcr擴增了中華絨螯蟹的sox基因，並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ，其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ，顯示該基因在進化上具高度的保守性。
  5. Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b. subtil is chromosomal dna ( from glyb to apre ), and 27 % homology with bacillus sp

    將此重組質粒phchi轉化巨大芽抱桿菌b . megateriumap25 ，得到兩株轉化子，分別命名為p25113一9 ， p25113一10 。
  6. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  7. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  8. A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron

    根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。
  9. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  10. The predicated amino acid sequences contain two conserved functional domains : a dna - binding domain ( hmg - box ) and a transactivation domain. the conserved motif of group b homology lies immediate adjacent to c - proximal region of the hmg - box. similar structure and identity of sox2 gene among mammals, birds, amphibians and reptiles suggest that sox2 gene have evolutionary conserved roles

    進一步分析表明，已巴灘2編碼的238個氨基酸多膚鏈含有兩個保守的功能區域:位於n端的hmg盒區（ 1一78aa ）和位於c端的轉錄激活區（ 131一236aa ） ，以及一個保守的特徵性b亞族同源區域（ 79一90aa ）與hmg盒區的c端相連。
  11. Relatively, the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96. 8 % - 97. 2 % at dna or aa levels, than the homology among hk46 and 3 strains, strains gx8 / 99 more than 98. 4 % - 98. 6 % at dna or aa levels

    為研究病毒的核酸分子結構與其致病性的關系，本研究選取了在致死率上不同的4個ibdv野毒株，比較了它們的vpz基因高變區共494個堿基序列。
  12. The orf1 proteins of all four chinese senv isolates had two motifs ( ftl and yxxk ) which were conserved in replication - associated proteins. in all four chinese senv isolates, the putative non - structure orf2 protein had a cav - like region. the putative orf3 protein had a serine - rich tract preceded by a cluster of basic amino acids ( r and k ). database scaning suggested this region had high a percentage of homology with dna topoisomerase i protein of d. melanogaster, and might play an important role in the replication of such single - stranded dna viruses

    所有這四個中國分離株，均保留了o即1中與復制有關的保守序列（ ftl和yxxk ） :在推測是非結構蛋白的orfz中均含有cav樣保守區w 『 x7一h一x3一c一xl一c一xs一h ; orf3蛋白的堿性氨基酸（精氨酸和賴氨酸）簇后緊接著一個富含絲氨酸的區域，與d . molanogaster的dna拓撲異構酶i有一定的同源性，推測在單鏈dna的復制中起作用。
  13. The sds - page electropheresis of whole - cell proteins was applied in classification of 71 strains isolated from astragalus spp. it was showed that the technique is a simple and rapid method in classification of rhizobia. the similarity of strains in the same group is 78 %, and dna homology is above 70 %

    採用sds page技術對71株黃芪根瘤菌進行了全細胞蛋白的聚類分析.結果表明，這是進行根瘤菌分類時一種簡便快速的分群方法，分群的菌株相似性水平為78 ，群內菌株的dna同源性70
  14. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  15. The nucleotide sequences of its dna of the gxcr1 shared more than 95 % homology with 102 strains of p. spp

    該菌的its核苷酸序列與102株青黴菌的its核苷酸序列有95以上的同源性。
  16. Four colonies of transformed e. coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained. the gene fragment in these isolates was identified by the methods of plasmid processing. dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b. subtilis from 2599451 to 2812870 was 85 %, and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b. subtilis ) in genebank

    測序並序列比較結果表明該基因片段同已發表的枯草芽孢桿菌幾丁質酶和內切葡聚糖酶編碼幕因的克隆及重組芽抱桿菌的構建glyb一apre之間的同源性是最高的，為35 % ;同bacz 』了了ussp . bp23ce1b 、 b . p朋刀us內切葡聚糖酶和b . pol理vxap一1 ， 4一內切葡聚糖酶的編碼基因的同源性只有27 % 。
  17. Using random primed gene walking pcr ( rpgw - pcr ), ctsox2 gene was cloned and sequenced from the digested genomic dna of trionyx sinensis. compared with the mouse and chicken sox2, ctsox2 shared 86 % and 91 % nucleotide homology respectively, and 93 % and 97 % amino acid identity respectively

    天i消化過的中華鱉（ trio矛移優sinensis ）基因組dna中克隆了c眨治xz基因（ 72obp ） ，它與鼠、鳥的及戲2基因分別有85 %和91 %的核昔酸同源性， 93 %和97 %的氨基酸同源性。
  18. Strain 10010 belong to nocardiopsis, the sequence homology with n. lucentensis was 98. 80 %. the two strains were similar to n. lucentensis. but they were different in colony colour, sugar of whole cell, menaquinones characteristics and dna g + c mol %. so they are maybe different species

    菌株10001與菌株1001016srdna序列相似性為99 . 58 ，但菌株10010和菌株10002菌落顏色不同，且在細胞壁糖型、醌類及dnag + cmol等方面有差異，可能為不同種。
  19. I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method, ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid, the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e. coli. the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses

    4 . h隊b一p及其突變體高效表達工程菌的構建:將用pcr擴增的h隊b一p 、 h獄b一p35 、 h隊b一p34基因，分別與pinpointxa一3質粒連接，轉化jm109大腸埃希菌，獲得的工程菌在表達融合蛋白時不穩定，第5代后即見不到表達條帶。
  20. But the comparison amino acid sequence shows that gpv dna has high homology with the human adeno - associated virus aav - 2 in dependovirus and b19 in erythrovirus

    Gpv與依賴病毒屬成員aav - 2及紅病毒屬成員人細小病毒b19的進化關系較近。
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