dna hybridization 中文意思是什麼

dna hybridization 解釋
dna雜化
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • hybridization : 混成作用
  1. Double cross - over strains aved24 and avec9 were obtained after growth of several generations on mym plate with or without antibiotics selection. genomic dna of double cross - over strains aved24 and avec9 were extracted and southern hybridization were performed

    分別提取同源雙交換茵株aved24和avec9基因組dna ，通過薩瑟恩（ southern ） dna印跡雜交，結果證明l
  2. Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant

    本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。
  3. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  4. Finally, the hybridization on the 3 - pyrrole - dna polymer modified electrode was detected with methylene blue ( mb ) as a hybridization indicator, thus the function of 3 - pyrrole - dna modified electrode was qualitatively analyzed

    實驗結果證明， mb可以用作無標記的dna雜交檢測；同時表明，電聚合法製作的dna晶元是有檢測功能的。
  5. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  6. Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization, the author studied many fingerprints of the same dna, the different organic fingerprints of the same organism and fingerprints of parent and offspring. the patterns were completely different among the different strains and those of the samples from the same strain were completely identical

    採用公安部二所自行研製的jl - 02多位點探針對5個品系的近交系小鼠和2個品系近交系大鼠進行了dna指紋分析，經過對同一dna的反復製作dna指紋圖和同一個體不同組織進行的dna指紋圖製作及對親代和子代（同品系內和不同品系間雜交）間的dna指紋圖比較。
  7. The signal provided by the probe is a measure of the degree of hybridization. traditional dna probes are labeled with radioisotopes i. e. 32p, i25i, 3h or 35s

    傳統的dna分子雜交採用的是放射性標記的檢測方法，這種方法雖然靈敏度高，但存在放射性物質對人體及環境的危害。
  8. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  9. Due to these inherent advantages, ecl method has attracted much attention from all analytical fields, especially from biochemical analysis. in this dissertation we focused on the preparation of a new type of dna probes which were labeled with ecl activated substances. based on coupling with the dna hybridization and immobilization techniques, we have developed new ecl methods for the determination of special dna sequence

    本論文通過研究了多種ecl活性物質的發光性能，並以這些物質為標記物制備了多種高靈敏度的dna - ecl探針，結合dna雜交技術和dna固定化技術，將高靈敏度的ecl檢測手段應用於生命物質dna的序列識別及含量測定，為dna傳感器的研究和基因晶元的開發提供了新的思路和方法。
  10. 705bp dna fragment of mxnrampl gene and full cdna of mxlrtl gene which were related to resist iron stress were cloned by using malus xiaojinensis cheng et jiang - the first iron - efficient genotype in the genus malus in the world as material. ( 1 ) using fragment of nramp gene from wheat and fe ( ll ) - transporter gene fragment of maize ( zmlrt ~ ) as probes, we analysed these genes by blotting hybridization technique in malus xiaojinensis cheng et jiang

    本實驗以中國農業大學園藝植物研究所篩選到的一個蘋果鐵高效基因型? ?小金海棠（ malusxiaojinensischengetjiang ）為試材，分別克隆了小金海棠的抗缺鐵相關基因mxnramp1基因的752bp基因組dna片段和fe （ ） -轉運蛋白基因（ mxirt1 ）的cdna全長，為深入探討小金海棠抗缺鐵的分子機理奠定了基礎。
  11. The equipment here may be pipette, electrophoresis system capillary, transblot system, chest / oven molecule hybridization, water bath, dna sequencer, microplate - reader for elisa, microplate washer and so on

    本區所使用的儀器設備可能有加樣器、電泳儀（槽） 、電轉印儀、雜交爐或雜交箱、水浴箱、 dna測序儀、酶標儀和洗板機等。
  12. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  13. Pdms - glass microchip used for performing dna hybridization reaction. has been fabricated and studied

    製作並研究了pdms晶元上的dna分子雜交反應。
  14. Biotin - avitin system ( bas ) was used to immobilize dna on the pdms channel compared to the direct immobilization methds. the hybridization reaction also has been examined between immobilized probe and target dna

    針對pdms新型材料，試驗了兩種dna分子的固定方法，確定了生物素?親和素介導的dna分子固定化方法，並使用此晶元和固定方法進行了晶元上的dna雜交反應。
  15. By sun, bo ( major : marine biology ) directed by professor zhang, guofan and dr. liu, xiao population genetics of four argopecten irradians cultured populations was analyzed by using random amplification polymorphism dna ( rapd ) techniques. and partial dna sequences of the internal transcribed spacers ( its ) from four representative samples ( one sample represent for each population ) were amplified by pcr and sequenced in order to stress the phylogenetic relationships of the four populations. besides, by rapd technique, two argopecten irradians families were studied to examine whether the hybridization experiments were successful and which family was more suitable to be materials in future genetic linkage map construction

    本文以海灣扇貝4個養殖群體為研究對象，採用隨機引物多態性dna （ rapd ）技術進行了群體遺傳學研究，並嘗試使用4個群體中代表個體的內轉錄間隔區（ its ）序列比對分析結果作為參考；另外，本文還運用rapd技術對人工異體交配的2個海灣扇貝家系的雜交成功率進行了分析，均獲得成功證實，並評估了兩個家系構建遺傳連鎖圖譜的潛力。
  16. From the results of the experiment, we can see that simultaneity electrochemical polymerization of 3 - pyrrole - dna and 3 - [ 2, 5, 8, 11 - tetraoxa - tridecyl - 13 - ol ] - pyrrole was a new promising method for preparing dna chips because this method had such following advantages : first, the immobilization procedure can be controlled easily. second, with the progress of microelectronics, the miniaturization and integraterization of polypyrrole - based dna chip can be achieved in the future ; third, using an electrochemical active compound like the mb, the hybridization detection will be performed more easily

    從以上實驗結果可看出，電化學聚吡咯方法制備dna晶元和傳統的方法相比具有下列優越性：首先，固定可通過電化學信號控制和完成，操作簡單易行；其次，微電子技術和電化學的進一步結合，可以提高晶元的集成程度，將dna晶元進一步向產業化方向推進；第三，通過應用電化學活性物質，有望建立無標記的dna晶元檢測技術，有利於減少環境污染、提高靈敏度、降低成本。
  17. In this dissertation, the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna, respectively

    本實驗以中國倉鼠卵巢細胞（ cho ）為實驗材料，利用基因轉染、熒光原位雜交並結合激光共聚焦顯微鏡觀察的方法，在dna和rna水平上分別對rna聚合酶的轉錄位點和轉錄子的分佈進行了檢測。
  18. The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area, which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis

    而16srdna克隆文庫分析和dna - dna雜交的結果表明「結核」區沉積物中這兩類細菌數目很少，說明「暖池」區沉積物中的微生物群落結構特徵是對環境因素的一種響應，同時也可能是影響該海區深海及海洋環境的一個重要因素。
  19. The 7 isolates were identified based on physiological and biochemical characterization, 16s rdna sequence, g + c mol % and dna - dna hybridization. isolate tl was identified as bacillus cereus. isolate gl, c4 and c5 were identified as bacillus megaterium

    在上述試驗的基礎上，本文分別用生理生化性狀、全細胞脂肪酸成分分析、 16srdna序列分析及dna - dna雜交實驗對這7個含有nifh的菌株進行了鑒定。
  20. Other groups were identical phylogenetic groups. to determine the exact taxonomic position and phylogenetic position of the rhizobia isolated from the root nodules of pueraria spp, the dna g + cmol % test, dna - dna hybridization and 16s rdna full sequence must be studied

    數值分類、 ypfcr分析、 16sn3napcr rflp分析對慢生葛藤根瘤菌的分群存在較大差異，說明葛藤根瘤菌內豐富的多樣性和分類地位的復雜性，要確定其確切分類地位和系統發育關系，要通過g cmol含量測定、 dna
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