dna marker 中文意思是什麼

dna marker 解釋
dna標準
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • marker : n 1 作記號的人;打分數的人;記分器;劃線器;指示器;【無線電】指點標;(撞球等的)記分員。2 【軍...
  1. Application of dna molecular marker in deciduous fruit tree

    分子標記在果樹上的應用
  2. Application of microsatellite dna as molecular genetic marker

    在分子遺傳標記研究中的應用
  3. A pcr - based dna marker for a - gliadin gene in common wheat

    醇溶蛋白基因的分子標記
  4. The chilopoda and diplopoda are monophyletic group respectively. although there are some limitations, the mitochondrial dna as a model system is being used as a powerful tool in phylogeny analysis. it is the only molecular marker that can be used in the phylogenetic studies at genomic level

    線粒體dna基因組全序列作為研究動物系統發生的模式系統,雖然有一定的缺陷,但仍是生物學家研究系統進化最有力的工具,它是目前唯一可以提供基因組水平上進行系統發生研究的分子標記。
  5. Frankfurt os, robb ja, sugar baker ev, et al. monoclonal antibody tosingle2stranded dna is specific and sensitive cellular marker of apoptosis [ j. exp cell res, 1996, 226 ( 2 ) : 3872397

    吳國慶,李少華,徐志偉,等.抗單鏈單克隆抗體雜交瘤細胞株的建立及鑒定[ j ] .細胞與分子免疫學雜志, 2002 , 18 ( 5 ) : 4792480
  6. We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment

    本研究從分子生物學角度入手,採用cytb作為分子標記,採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ,通過pcr技術,共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。
  7. Second, genomic dna polymorphisms of three mandarinfishes populations ( each bulked dna pool composed by 10 individuals ) were tested by rondom amplified polymorphic dna ( rapd ) using bulked segregant analysis ( bsa ). 12 primers selected from operon kitz yielded reproducible and polymorphic dna fragments ranging from 125bp to 3kb. these primers generated 60 scorable marker bands of which 15 ( 25 % ) were polymorphic in qiupu river population ; 59 bands in changjiang population, 15 ( 25. 4 % ) were polymorphic ; and 58 bands in wanfuhu mandarinfishes, 19 ( 32. 8 % ) were polymorphic

    20個引物中有12個在三群體間產生多態性,這12條引物在秋浦河鱖魚中擴增出了60條帶,其中15條為多態性(佔25 ) ;長江鱖魚59條帶, 15條為多態性(佔25 . 4 ) ;萬佛湖鱖魚58條帶, 19條為多態性(佔32 . 8 ) 。
  8. Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal

    本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究,並與生化位點標記分析法進行比較,旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法,為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。
  9. Z which involve in melanin, tyrosinase related protein 1 gene ( tyrpi ) and dermal melanin inhibitor gene ( id ). the genomic structure of tyrp1 was determined and its correlation between melanin accumulation and tyrp1 expression was studied. moreover, we constructed a bac contig near id locus which was on a region short of dna marker in chr. z

    利用構建的bac文庫對z染色體上黑色素相關基因酪氨酸酶相關蛋白1基因( tyrp1 )的基因結構以及該基因的表達與黑色素沉積的關系進行了研究,同時構建了位於z染色體上缺乏標記區段的表皮黑色素抑制因子基因( id )的bac重疊群。
  10. Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker

    將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起,置於兩個同向的解離區之間,再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。
  11. Dna genetic marker identification is a new method based on the identification of chinese drug and molecular biology

    Dna遺傳標記鑒定方法是在中藥四大鑒定方法及分子生物學發展的基礎上衍生出來的一種新的鑒定法。
  12. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ,分別編碼501 , 333 , 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ,而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ,復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上, 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳,對受體菌株無明顯不良影響。
  13. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒(脾淋毒)基因組cdna文庫的構建、序列分析:根據已發表的豬瘟病毒( csfv )核苷酸序列,藉助計算機軟體分析,選擇高保守區段和基因組中的單一限制性酶切位點,利用rt - pcr及nested - pcr和helf - nestedpcr技術,成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ,分別克隆到pmd - 18t或pgem - teasy載體進行測序后,拼接出了其核苷酸序列。
  14. Tandem repeats play a variety of roles in gene expression, regulation and evolution, and are ideal marker in genetic mapping. further more, the techniqe of dna fingerprinting based on the polymorphism of tandem repeat is now widely used in various fields such as medical jurisprudence, etc. the more eye - catching discovery is, in recent years, that some genetic diseases are related with certain trinucleotide repeats

    串聯重復在基因表達、調控和遺傳等方面起著十分重要的作用,同時它因具有高度多態性,已成為基因組遺傳圖譜和物理圖譜的理想界標,另外以串聯重復為基礎的「 dna指紋技術」在法醫學等領域廣泛應用。
  15. Abstract : this paper reviewed the recent advance of the application of dna molecular marker in various aspects of tomato breeding including genetic map construction, germplasm research and purity control of cultivars, identification markers linked to important genes and map - based gene cloning

    摘要本文綜述了dna分子標記在番茄遺傳圖譜構建、番茄種質資源研究與品種純度的鑒定、番茄基因分子標記研究及番茄基因圖位克隆方面的應用研究進展。
  16. 2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region

    以增強的ta29啟動子驅動克隆的coda基因,構建成花藥特異性嵌合基因表達盒;將此表達盒插入雙元載體p3301 ,構建成以ppt抗性基因為選擇標記,以gus為報告基因的植物表達載體。
  17. Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced

    在一步轉化系統中,光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求,並且簡化、優化了整個共培養過程,省去了一篩、二篩和預分化步驟,只用7周的時間就可以初步得到再生轉化植株;共191塊愈傷組織得到125塊抗性愈傷組織,轉化頻率達到65 . 4 ,最後共得到99棵獨立來源的再生植株,抗性愈傷組織再生頻率達到79 . 2 。
  18. 3. the discrimination of 24 wild tea germplasm resources using dna markers was conducted. the result showed that rapd marker was a very effective tool and robust method in tea germplasm discrimination

    3 、對原產于雲南等地的24份茶樹資源進行分子標記鑒別研究,表明rapd標記在鑒別茶樹資源方面非常有效。
  19. Over the past 5 years, professor lo and his colleagues have shown that plasma dna is a powerful prognostic marker for survival following radiation therapy for nasopharyngeal cancer

    盧教授還研究其他血漿核酸,例如癌癥病人血漿中的腫瘤核酸。在過去五年裡,他證明血漿dna是鼻咽癌放射治療后一種強而有力的預測標記。
  20. These strategies involved marker - assistant selection, map - based cloning, gene silencing and dna chip technology

    這些新策略的實施,將運用分子標記及其輔助育種、基因圖譜克隆、基因沉默以及基因晶元技術等dna操作技術。
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