dot blot 中文意思是什麼

dot blot 解釋
斑點分子雜交法
  • dot : n 多特 〈Dorothea 的昵稱〉。n 1 點;圓點;句點;【音樂】附點〈音符后的一點,表示延長1/2拍〉。2 一...
  • blot : n 1 墨污,墨漬,污點,污斑。2 瑕疵;恥辱,污名。3 〈古語〉塗去,抹去。vt ( tt )1 用(墨水等)弄...
  1. Bl21. the dot blot hybridization verified that the scfv was secreted to the medium

    從培養基中可檢測到可溶性抗人絨毛膜促性腺激素的單鏈抗體。
  2. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後,用地高辛隨機引物法標記成momp基因核酸探針,斑點雜交檢測衣原體基因組dna ,靈敏度可達10pg ,且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較,初步證明該探針具有較高的敏感性與較強的特異性。
  3. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查,本研究採用原核表達系統( gst融合表達系統)表達tgev纖突蛋白( s蛋白)中含有b和c抗原位點的多肽,並且制備了非放射性地高辛標記的核酸探針,通過斑點雜交( dot - blot )檢測tgev核酸rna 。
  4. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析,結果表明: iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性,兩者之間僅相差4個核苷酸,同源性高達99 . 7 ,從而證實了iltvtk基因是高度保守的,為iltvtk基因核酸探針的制備提供了有力的依據。
  5. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達,用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達,對表達上清處理后,用sds - page和western - blot進行鑒定。同時,用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  6. Protocol of dot blot and in situ hybridization for white spot virus

    對蝦白斑病毒斑點雜交和原位雜交檢測操作規程
  7. Dot blot mccallum mccallum splaques

    斑點分子雜交法
  8. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    ( 3 )細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣,細胞核轉化獲得了生長良好的抗性萵苣植株,經pcr 、 pcr - southern 、 southern斑點雜交分析證實, adw基因已整合到萵苣基因組中。
  9. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達:分別將csfv兩個代表株的e2基因克隆入畢赤酵母( p . pastoris )分泌型表達載體ppic9k中,酶切線型化后電穿孔導入p . pastotis進行整合,經g418篩選得到25個高拷貝轉化子,經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  10. Dot blot and northern blot studies revealed that amphihmgb expression is restricted mainly to ovaries and guts in adult amphioxus while it is highly expression during embryogenesis

    文昌魚amphihmgb蛋白不含c ?端酸性尾巴,為一堿性蛋白,等電點為9 . 88 。
  11. Gene silencing, a common phenomenon in phb production through nuclear transformation, had n ' t been observed in the chloroplast transgenic plants analyzed by northern dot blot

    Northern點雜交檢測表明與phb合成相關的三個基因均能在轉錄水平表達,未出現核轉化中經常發生的「基因沉默」現象。
  12. Twentymo transgenic plants were selected by dna dot blot analysis, 15 of them detected hybridization signals and the positive rate was 68. 2 %

    通過dnadotblot分析,對22個轉化株進行篩選,其中15株檢測到雜交斑,陽性率為68
  13. The activity of the antiserum was tested by dot enzyme immunization assay ( diba ) with purified antigen. western blot of hela nuclear protein extract, which contained natural hbaf53, showed that the antiserum is also a specific antiserum to natural hbaf53. therefore the highly specific and sensitive antiserum can be applied to various studies

    用純化的抗原蛋白,經斑點印跡試驗測得baf53抗血清的效價,又用提取的hela細胞核蛋白(含有天然baf53蛋白)進行免疫印跡分析( westernblotting ) ,證明天然baf53蛋白也是該抗血清的抗原,說明獲得的多抗血清具有高特異性和敏感性,可用於多方面的研究。
  14. Application of pcr - reverse dot blot in genetic diagnosis of - thalassemia

    地中海貧血基因診斷中的應用
  15. To screen the library, defferential screening had been employed. sixty - four clones were randomly picked to perform dot blot

    隨機挑出13個克隆進行限制內切酶分析,結果有10個質粒含有300一600bp的插入片段。
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