dot blotting 中文意思是什麼

dot blotting 解釋
點漬法,斑點印跡
  • dot : n 多特 〈Dorothea 的昵稱〉。n 1 點；圓點；句點；【音樂】附點〈音符后的一點，表示延長1/2拍〉。2 一...
  • blotting : 擦去油污
  1. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  2. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  3. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  4. Another detection method, dot blotting, was built also based on 35s promoter and nos terminator. the two genes were labeled by digoxin to done as probe used in dot blotting

    並將35s啟動子和nos終止子用地高辛標記后制備成基因探針，建立了轉基因檢測的分子雜交技術?點雜交。
  5. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  6. Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

    具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。
  7. [ methods ] two phage - epitope libraries were screened with two anti - vegf neutralizing monoclonal antibodies - jh121 and vg189, the clones were tested by dot blotting and elisa

    並通過硝酸纖維膜斑點印跡法進行陽性克隆鑒定。 elisa分析陽性噬菌體克隆與抗體的親和力。
  8. Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig. the result showed that the expression of arf gene was mainly in anthers of sterile and fertile, pollen of fertile, corolla rather than in leaves, radicel and ovule

    棉花ari ； 』基因的表達分析用地高辛將棉花arf基因標記為探針，與棉花洞a的不育株花藥、司有株花藥、可育花粉粒、葉片、花冠、胚根和胚珠的總rna進行斑點雜交。
  9. The expression of target protein was identified with dot - elisa, sds - page and western blotting

    Elisa 、 sda page 、 western blot等方法在蛋白水平對ctla4胞外區蛋白的表達進行了鑒定。
  10. The fusing gene was transformed into leaf tissue of tobacco cultivar nc89. more than 100 plants were obtained. transgenes were confirmed to be inserted into tobacco genome of r0 generation by pcr and dot - blotting analysis

    通過kpn位點與成熟番木瓜蛋白酶的cdna連成一個融合基因，並插入到pbi121的原gus位點，構建成nib -番木瓜蛋白酶融合基因的植物表達載體pnpa 。
  11. The dna and rna dot blotting revealed transcription of the polyprotein gene into mrna in the vero cell

    比較了dna疫苗和兩種常用的弱毒苗（ b87 、 d78 ）的安全性和免疫效力。
  12. It indicated that 76. 5 percent of positive frequency of all the plants was achieved by using southern dot hybridization, 87. 5 percent of the plants with gus positive activity was gotten by pcr southern blotting and 42 percent of the plants with gus positive activity was gotten by southern blotting

    轉基因卡那黴素抗性植株中， gus表達陽性率為43 ，隨機抽樣的方法進行殺蟲肽和npt基因的pcr檢測陽性率均為100 。經過分子生物學檢測表明， southern斑點雜交76 . 5植株表現陽性。
  13. By northern dot blotting, it also showed that the foreign gene could express in the transgenic plants

    Northern斑點雜交表明，外源基因在轉基因植株中正常表達。
  14. The results of our study of last year found that the secretion levels of ley and mmp - 9 of the same morphology were different. by using highly sensitive dot blotting method, the difference can be clearly detected

    我室在近期研究發現不同的胚胎雖然形態上看沒有區別，其le ~ y 、 mmp - 9的分泌卻不相同，這種差異可以用點雜交的方法檢測到。
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