double strand rna 中文意思是什麼

double strand rna 解釋
雙鏈rna
  • double : adj 1 兩倍的，加倍的。2 雙的，二重的，雙重的；對，雙，兩，復。3 雙人用的；折疊式的。4 （意義）雙...
  • strand : n 1 〈詩〉（海、湖、河等的）濱，岸，灘。2 〈the S 〉 （倫敦的）河濱馬路。vt vi 1 （使）（船等）觸...
  • rna : RNA ＝ribonucleic acid 【生物化學】核糖核酸。
  1. Poly ( a ) + rna was isolated from the salt - induced aloe vera l. leaves as tester and the normal aloe vera l. leaves as driver, and single - strand cdna and double - strand cdna were sythesized in turn

    乙， ）和鹽敏感品種皂質蘆薈（ a了。 。胭p助打邵施叫為材料，進行了northernblotting和酶活測定。
  2. Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis. the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation, 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain. referred to the published sequence two primers were designed and synthesized

    給4周齡spf雛雞人工接種雞傳染性法氏囊病病毒hb - bp毒株， 48小時后採取法氏囊，用雙licl分級沉澱法提純全長基因組dsrna ，設計一對引物，通過rt - pcr方法進行了體外擴增，獲得hb株a節段基因全長cdna 。
  3. To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase

    方法：根據端粒酶htert基因1573 ? 1591位的核酸序列，構建帶t7啟動子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。
  4. Conclusions : the in - vitro method that partial double - strand dna with t7 wi l. - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield, excellent purity shrna. lt is a convenient -, effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory

    結論：以帶t7啟動子部分雙鏈dna為模板，用t7rna聚合酶體外合成出的shrna產量較高，純度較好，是一種簡便、高效、低成本的短鏈rna的制備方法，適合於普通實驗室用來進行短鏈rna的合成和rna干擾實驗。
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