e (electrophoresis) 中文意思是什麼

e (electrophoresis) 解釋
電泳
  • e : （pl E s e s ）1 英文字母表第五字母。2 【音樂】E調，E音。3 E字形。4 〈美國〉（順序）第五等，（成...
  • electrophoresis : n. 電泳（法）。
  1. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。
  2. The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd

    4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。
  3. The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods

    根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。
  4. Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction, fraction with ( nhi ) 2864, hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr. cml agglutinated type a human red blood cells specially. the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis

    野花生豆（ crotalariamucronata ）經磨粉、浸取、硫酸銨分級沉澱、豬胃粘蛋白- sepharose4b親和層析、 sephacryls - 200hr分子篩層析可得到一表觀分子量為103kd且對a型血紅細胞專一凝集的野花生豆凝集素（ crotalariamucronatalectin ， cml ） 。
  5. In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226

    將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。
  6. In order to understand the epidemiological behaviors of this pathogen, we studied strains e. coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city, jiangsu province, by methods of pcr, antimicrobial susceptibility, biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal

    為了探索我國大腸桿菌o157 ： h7感染的流行病學特點，我們使用了聚合酶鏈反應、生化反應、抗生素敏感實驗、脈沖電場凝膠電泳等分子生物學方法，對我國1999 - 2000年徐州地區分離到的部分大腸桿菌o157 ： h7進行了分析。
  7. The e2 gene was amplified by rt - pcr, then examined the fragment by electrophoresis. after purification and insertion into pucm - t vecter, the recombinant plasmid pbne2pi and pbne2pii were obtained. then they were transfected e. coli jm109 and screened positive clones by blue or white plaques. the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis

    通過rt - pcr擴增e _ 2基因，電泳測定擴增片段的大小，經純化后，連接于pucm - t載體，獲得重組質粒pbne2p 、 pbne2p ，轉化e ? colijm109 ，經藍白斑篩選，挑取陽性克隆，提取質粒，直接電泳鑒定和酶切鑒定。
  8. 3. dna double strand breaks ( dsbs ) induced by ion beams of gel electrophoresis experiment has shown positive linear with dose, i. e., double strand breaks will increase as the dose increased

    分子生物學技術? ?電泳技術分析結果表明，離子輻射造成的dna雙鏈斷裂（ dsb ）與粒子劑量呈線性正相關關系，即隨著粒子劑量值增大， dsb量也逐漸上升。
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