echistatin 中文意思是什麼
echistatin
解釋
鋸鱗血抑-
Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。 -
Conclusion constructed the high - level expression clone of echistatin in e. coli. the expression of recombinant protein is higher, it make the further study of echistatin feasible
結論成功構建了echistatin的原核高效表達克隆，表達量高於現有國內外研究水平，為echistatin功能及相關疾病的研究奠定了基礎。 -
Results : designed and synthesized the coding gene of echistatin. and constructe its single copy expression vector, identify the vector by dna sequencing. but it could not express echistatin in e. coli. designed and synthesized the modified coding dna of echistatin, and constructe its two copy expression vector, identify the vector by dna sequencing, but it could not express echistatin in e. coli
結果設計併合成了echistatin編碼基因dna序列，構建了echistatin單拷貝表達載體，經dna測序鑒定正確后， sds - page及western - blotting結果表明： echistatin在上述菌株中未獲得高效表達。 -
Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
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