ecor 中文意思是什麼

A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。

Cut off beta fragment from plasmid prok. ii with hindlll and ecor i as insert, and cut pa into linear plasmid as vector fragment. link the insert and vector fragment together with t4 ligase, and the new vector with gene beta and gus was constructed

用hind和ecor雙酶切prok質粒，獲得beta基因片段作為插入片段，用hind和ecor雙酶切a質粒作為載體片段，將插入片段與載體片段相連，即構建成含有beta和gus的雙基因載體。

Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。

Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。

By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells

將得到的序列在genbank和embl數據庫中進行了同源比較，結果顯示：本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異，編碼的氨基酸有3個氨基酸殘基的差異，同源性為98 ，影響蛋白質高級結構的保守二硫鍵為2個。

Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。

The aflp fingerprinting of 20 seedlings of m. xiaojinesis with 8 ecor i - mse i primer combinations showed that m. xiaojinesis in natural population had a high degree of genetic identity, its genetic similar coefficient being 98. 92 %

用8對ecor - mse引物組合對小金海棠家植實生群體內的20個株系進行了aflp分析。

That changes of the economy profit for several primary crops were accompanied with the progress of structure adjustment indicated that the ecor. omy profit is the main driving force to the structure adjustment

林地的經濟效益基本為負值。仔細分析發現，它的經濟功能弱，但其生態和社會功能較強，因此，農牧戶自營普遍。

One solobp ecor i fragment containing phospholipase gene was isolated. further sequence analysis and subcloning revealed a 963bp phla. gene coding a 320aa phospholipase phl with deduced molecular weight of 33kd

通過測序及亞克隆分析，發現一個磷酯酶的基因phla ，長度為963bp ，預測編碼一個由320個氨基酸組成，分子量為33kd的磷酯酶phl 。

I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。

The fragments digested with restriction endonucleases ecor i and hind iii were subsequently cloned into the vector pkk223. 3. e. coli jm 109 transformed with the expression plasmid was grown in m9 media to an optical density of 0. 7

把重組質粒轉化入大腸桿菌jm109中，挑取轉化成功的單克隆菌，接種到含amp的lb培養基中，於37振搖過夜。

First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。

According to the nucleotide sequence of selected antigen epitope, pcr primers supplemented with the ecor i and sal i sites were designed

根據選出的抗原表位區的核酸序列設計pcr引物，並於引物末端添加ecor和sa11酶切位點。

Pfge analysis of 6 ecor ] digested bacs clones which were derived from the library and contained lmbr1 sequence proved the overlap among the bacs

對篩選得到的6個含lmbr1序列的bac克隆酶切分析的結果表明bac克隆之間是相互重疊的。

1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers

將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。

At the same time, puc18 vectors were digested by ecor i enzyme and their 5 ; ends were treated by ciap

同時以ecor酶切消化puc18質粒dna ， ciap對載體5端去磷酸化處理。

Polymorphism of bovine tlr2 gene digested with ecor v and its associations with somatic cell score

酶切多態性與體細胞評分的關聯分析

In this case, two restriction sites ( ecor i, hind iii ) were added to the primers ( p1, p2 ) separately

擴增產物經雙酶切后，重組到表達載體pet - 32a （ + ）中。

The 5 " and 3 " ends of the pcr product were respectively introduced into bamh i and ecor i sites

首先設計一對特異性引物，以pt7 - ri為模板， pcr反應擴增出ri基因，並在其上下游分別引入bamh 、 ecor酶切位點。

Among 42 ecor i - mse i primer combinations, fifty - one aflp markers correlated to fe - efficient trait were identified using bsa - aflp technique

用bsa - aflp方法確認了與鐵高效性狀相關的51個aflp標記，由28對引物組合獲得。