eiav 中文意思是什麼
eiav
解釋
馬傳染性貧血病毒-
Equine infectious anemia virus ( eiav ) is a member of the lentivirus of retrovirusae and the causative agent of equine infectious anemia ( eia )
馬傳染性貧血病毒( eiav ,簡稱馬傳貧)是反轉錄病毒科慢病毒屬的成員之一,是馬傳染性貧血病( eia )的病原體。 -
The chinese donkey leukocyte attenuated strain of eiav ( dla ) was the only successful lentivirus vaccine up to now, which has been applied extensively in china against eia
我國的eiav弱毒疫苗是迄今為止世界上唯一投入應用的慢病毒疫苗,成功地控制了我國eia的流行。 -
Chinese equine infectious anemia virus donkey - leukocyte attenuated strain ( eiav - dla ) is the unique lentivirus vaccine which has been used in large range for more then 20 years in china mainland
該病以間歇熱,貧血、消瘦為特徵,呈慢性進行性,終生持續感染,以死亡而告終,少數病例可發生急性死亡。 -
Equine infectious anemia virus ( eiav ), a macrophage - tropic lentivirus, causes persistent infections of horses characterized by rapid development of acute disease and following a long chronic and persistent phase
馬傳染性貧血病毒( equineinfectiousanemiavirus , eiav )是反轉錄病毒科慢病毒屬的重要成員,是引起馬屬動物傳染性貧血病的病原。 -
The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo, obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro
該毒株是將eiavl株( eiavl )通過驢體傳代,使其毒力明顯增強,然後在驢白細胞培養物上連續傳代致弱獲得的。 -
Because of difficulty in differentiating eiav wild strain from the vaccine strain ( dla ) in vivo , we have to build a fast identification method between wild strain and vaccine strain of eiav s2 is an accessory protein of equine infectious anemia virus ( eiav ), the s2 function is not fully defined
由於我國馬傳貧弱毒疫苗存在野毒和疫苗毒鑒別困難的問題,目前尚未得到歐美等發達國家的認證,如要進入國際市場,急需建立一種eiav強、弱毒快速鑒別診斷方法。 -
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上,構建了4個全長基因的分子克隆,分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ,其中兩個轉移到低拷貝載體plg338上,命名為plgd30343 、 plgd70344 。 -
The eiav - pok8. 2 - his was transfected into the donkey leukocyte culture. following an incubation period, reverse transcriptase activity was detected in cell culture supernatants. cytopathic effect was observed by no. six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8. 2 - his
提取前病毒dna , pcr擴增p1p4片段,亞克隆至pmd18 - t載體,測序證明前病毒dna含有六個組氨酸,從而在體外獲得了感染性克隆病毒株。 -
It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his
本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體( pok8266 )為模板,通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點,形成含有酶切位點( nspv )的突變體( p1p4 ) 。 -
The donkey - adapted strain of eiav ( dv116 ), parental strain of eiav - dla, is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s. the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period. in this study, we constructed a full - length provirus dna clone of dv116 by pcr
本實驗中將eiav驢強毒dv體外感染驢白細胞培養物,一定時間后收獲病毒(本文中簡稱dv116 ) ,提取eiav前病毒dna ,以pcr法分別擴增並克隆了包含全長基因的三段前病毒dna片段,以雙脫氧法測定了dv116病毒全基因序列共8236個核苷酸。
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