eif 中文意思是什麼
eif
解釋
埃爾夫-
We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm
Eif - 5a的hypusine修飾是其活性和功能發揮所必需的,我們通過pcr方法實現了hypusine位點的定點突變,並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。 -
The eukaryotic initiation factor 5a ( eif - 5a ) is one of the most abundant initiation factors in the eukaryote, and it is the only protein in nature that contains a hypusine residue
真核起始因子5a ( eif - 5a )普遍存在於所有真核細胞內,是目前發現唯一一個含有hypusine殘基的蛋白質。 -
Then protein crystal of eif - 5a was observed using atomic force microscopy. further, the polyclonal and monoclonal antibodies were gained from immunized rabbits and rats. hypusine - contained eif - 5a plays a role in cell growth and differentiation
用得到的重組蛋白分別免疫家兔和小鼠,獲得兔的抗血清和三株持續分泌單抗的雜交瘤細胞株,為進一步分析eif - 5a的功能提供了檢測手段。 -
It showed that eif - 5a subcellular localizat ion was a dynamic process, and it might be a nucleocytoplasmic shuttle protein. our study is the first to show that the inhibition of hypusine formation altered the subcellular distribution of gfp - eif - 5 a, and made it unable to localize at its proper site of action
然後利用氨基酸突變、添加阻斷劑等方法,抑制eif - 5a的hypusine修飾,觀察到eif - 5a始終為全細胞分佈,提示抑制hypusine的形成可影響eif - 5a的亞細胞定位,進而影響其功能的發揮。 -
And one day the iittie eif famiiy
有一天這個精靈家庭 -
Iittiest eif of aii
最不起眼的精靈 -
Eif at times the car does not respond immediately, try dialing again as the signal may be weak or the gsm network may be overloaded
如果遇到信號弱或gsm網路忙等情況,車輛可能不會作出迅速的反應。這時,請重新操作。 -
At first, the gene of eif - 5a was fished from mo - 7e cell line using rt - pcr. then we successfully construct the prokaryotic expression vectors of eif - 5a. after expression and purification, rheif - 5a was identified by western - blot and peptide mass - fingerprinting
本工作首先從mo7e細胞中提取rna ,通過rt - pcr方法釣取eif - 5a ,在確認序列正確的基礎上,構建eif - 5a的原核表達載體。 -
Further, eif - 5a overexpression through transfection promoted up inhibitor - induced apoptosis, and. mutation of eif - 5 a abolished the enhancing effect, we could conclude that the accumulation of hypusine - absent eif - 5a played an important role in the strong induction of apoptosis by up inhibitors
進一步的分析結果表明過表達eif - 5a ,可增大細胞對mg - 132的敏感性,而hypusine突變體無此現象,說明未形成hypusine的eif - 5a可促進阻斷泛素通路誘發的凋亡作用。
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