electrophoresis method 中文意思是什麼
electrophoresis method
解釋
電泳法- electrophoresis : n. 電泳（法）。
- method : n 1 方法，方式；順序。2 （思想、言談上的）條理，規律，秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
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Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。 -
Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page
將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。 -
We present a preliminary study on the zymograms of esterase ( est ), lactate dehydrogenase ( ldh ), malate dehydrogenase ( mdh ) from four groups of microtus fortis by vertical discontinous polyacrylamide gel electrophoresis method. the number, mobility and dye intensity of the enzyme band were analyzed
採用垂直板不連續聚丙烯酰胺凝膠電泳法對四類東方田鼠的酯酶（ est ） 、蘋果酸脫氫酶（ mdh ）和乳酸脫氫酶（ ldh ）進行了初步研究。 -
Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization
2 ）結果可靠，雜交特異性誘捕目的片段，同時去除了核酸中存在的pcr抑制物質，減少了因核酸提取不純造成的漏檢現象，結果輸出通過雙重判定保證了結果的可靠性。 3 ）靈敏度高，通過雜交進行結果判定，靈敏度比傳統的rt干cr大約高10倍。 -
The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods
根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。 -
Textiles. tests of fibres. method of determining the quantity of cysteic acid contained in hydrolysates of wool by electrophoresis on paper and spectrophotometry
紡織品.纖維試驗.用紙上電泳法和分光光度計法測定羊毛水解產物中磺基丙氨酸含量的方法 -
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
Activity of alcohol dehydrogenase and phenylalanine ammonia - lyase varied during the blooming of jasmine flower. the non - specific esterase isozymes were isolated by different methods. two enzyme stripes were gained with page ( polyacrylamide gel electrophoresis ) method, while eight stripes were obtained with ief ( isoelectrofocusing ) method
等電聚焦法分離茉莉花酯酶的效果較好，用聚丙烯酰胺常規分離只得到2條酶帶，而用等電聚焦法分離茉莉花酯酶同工酶可獲得8條比較清晰的酶帶。 -
In recent measuring method, protein electrophoresis is rapid, accurate, steady and economic, not affected by environment condition, we improve reactive system of our national tentative com protein gelatinous electrophoresis, and that which is more adapted to imaging process
在玉米種子純度檢測的方法中，蛋白質凝膠電泳法具有快速、準確、穩定、經濟、不受環境條件影響等特點，因此，選取此方法作為種子純度檢測方法。對國家試行的玉米蛋白凝膠電泳法的反映體系進行了改進，使得到的圖像更清晰。 -
Inspection of 10 - hydroxy - - decenoic acid in bee products for import and export - capillary electrophoresis method
進出口蜂產品中10 -羥基- -癸烯酸的檢驗方法毛細管電泳法 -
In the second part, polyphasic taxonomy methods such as morphological method, physiological and biochemical method, dna g + cmol %, soluble protein electrophoresis and 18s rdna sequence analysis were used for systematics of all strains isolated and purified with the method above
本論文第二部分採用形態學、生理生化特徵、 dnag + cmol 、可溶性蛋白電泳及18srdna序列分析等分類技術對所分離的16株甲真菌進行了系統的分類研究，從而初步確定了16株甲真菌的分類地位。 -
We aim for com breed of our province, using computer, pick - up camera, image gathering card, electrophoresis equipment ect as means, synthetically apply image processing technology, statistic theory and comparison experiment method, will combine the staining theory and and electrophoresis atlas character theory witch express character of seed vigor and purity with the image processing ; then develop a method and system of rapid measuring corn seed purity and vigor
本文旨在以我省的玉米品種為研究對象，以計算機、攝像機、圖像採集卡、電泳儀等設備為工具，綜合運用圖像處理、統計學理論和對比試驗等理論和技術，將計算機圖像處理技術與玉米種子純度與活力的生物學檢測方法相結合，研製出種子純度與活力快速測定的圖像識別與處理系統。 -
The detection of sod activity in native polyacrylamide gel electrophoresis ( page ) electrophoresis also showed the difference in sod activities among various bacterial strains and the bacterial strain 276 had the highest activity of sod, which was consistent with the result detected by photochemical method
利用聚丙酰胺凝膠電泳方法檢測sod的活性，其結果也表明不同細菌菌株的sod活性存在著差異，其中276菌株的sod活性最大，這與利用光化學方法檢測sod活性的結果是一致的。 -
And the field emission measurement was carried out at a pressure of 10 - 4pa. diamond powders coated with sliver are deposited on ito glass using electrophoresis coating method, through a solution of powders in isopropyl alcohol
利用葡萄糖的還原性將銀鹽中的銀還原出來，在超聲的條件下，還原出的銀將包覆在分散於溶液中的金剛石顆粒上。 -
A new method of two - dimensional electrophoresis is introduced, and applied to analyze protein subunits
摘要介紹了還原條件與非還原條件雙向電泳法，並應用此方法研究了種子蛋白質亞基結構。 -
One positive clone was obtained from the libraries with casein flat and electrophoresis comparison method. analyzed the sequencing result, the positive clone include the open read frame of mlap gene and upper regulatory sequence
序列測定分析表明，此重組質粒包含有長度為1377bp低溫堿性蛋白酶基因的完整的開放讀碼框架（ orf ）和上游基因調控序列。 -
A novel method is reported for mass separation of proteins, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis ( sds - page )
報導了一種基於十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）的新型蛋白質質量分離方法。 -
Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis. the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method
用脈沖電泳將經過同源重組的修飾的yac克隆分離出來通過脂質體轉化植物的原生質體，希望得到在植物細胞中穩定存在的植物人工染色體。 -
Standard test method for determination of dissolved inorganic anions in aqueous matrices using capillary ion electrophoresis and chromate electrolyte
用毛細管離子電泳和鉻酸鹽電解液法測定含水物質中溶解的無機陰離子的標準試驗方法 -
2. 4 preparation of yeast telomerase, the activity of telomerase was examined by the method of trap, polyacrylamide gel electrophoresis and silver staining
2 . 4酵母端粒酶的制備，端粒重復擴增法（ trap ） 、聚丙烯酞胺凝膠電泳及硝酸銀染色法檢測端粒酶活性。
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