elisa assay 中文意思是什麼

elisa assay 解釋
酶聯免疫吸附法,酶連接免疫吸附劑測定
  • elisa : 埃莉薩
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. Intestinal trefoil factor ; enzyme - linked immunosorbent assay elisa ; clone and expression

    小腸三葉因子酶聯免疫吸附檢測克隆表達
  2. Temporal patterns of vitellogenin ( vg ) synthesis and uptake as well as vitellin ( vn ) degradation in n. vitripennis, were studied using the method of enzyme - linked immunosorbent assay ( elisa )

    採用elisa測定法檢測了麗蠅蛹集金小蜂卵黃原蛋白合成與攝取，及卵黃磷蛋白降解的時間動態。
  3. It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ). the coefficient of correlation is significant and the potency is more than 1 : 800

    經間接elisa法檢測，抗體能與gst - cpti 、 cpti蛋白特異性結合，其相關系數達到顯著水平，效價1 800 。
  4. The enzyme - linked immunosorbent assay ( elisa ) and high performance liquid chro - matography ( hplc ) analysis for detection of mc were optimized. the removal rates of mc by conventional water treatment processes were investigated through the laboratory study and the detection of mc in every process in meiyuan drinking water treatment plant. results showed that the prechlorination of eutrophic water led to the release of intracellular toxins to water phase

    本文完善了mc的elisa和hplc分析方法，通過模擬試驗及水廠實測調查了富營養化太湖水中mc在常規凈水工藝中的去除特性，結果表明預氯化使藻細胞內的mc釋放出來，混凝沉澱對細胞外mc無去除作用，砂濾可去除17 . 2 40 . 4的細胞外mc和19 . 0 36 . 6的總mc ，加氯消毒對細胞外mc和總mc的去除率分別為30 45 . 3和30 51 . 7 。
  5. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  6. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  7. 5. the mpcr - rflp assay was useful for its reliability in monitoring hbv ymdd mutants. melting curve assay and pcr microplate hybridization - elisa assay should be further improved to increase their sensitivity and specialty

    5 . mpcr一rflp法檢測ymdd突變株具有較好的可靠性和可行性，是監測拉米夫定耐藥株的一種非常有效的方法;熔解曲線法和pcr微板核酸雜交法需要進一步完善以提高敏感性和特異性。
  8. The equipment shall be composed of : microplate reader for elisa ( or analyser elisa, analyser enzyme fluorescent assay ), microplate washer, micropipette, water - bath or temperature cabinet, refrigerator, shaker for microplate. etc

    儀器設備組合應為：酶標儀（或酶免分析系統、酶免熒光分析儀等） 、洗板機、加樣器、水浴箱或溫箱、冰箱、酶標板振蕩器等。
  9. The content of hbsag in blood by enzyme linked immunosorbent assay ( elisa ) is compared with the method of irradiating on blood of mice by pdt, the results show that the content of hbsag declines in various degrees and it can be concluded that inactiving hbv in blood by pdt may be realized

    實驗表明轉基因鼠血液中表面抗原的數量有不同程度的下降，從而證明血液照射光動力療法對血液中乙肝病毒具有滅活作用。
  10. Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province. 2. the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough

    應用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現病毒學反跳時的血清進行ymdd突變株的檢測，並與測序法和mpcr - rflp法比較它們的敏感性、一致性。
  11. A dot - enzyme - linked immnosorbent assay ( dot - elisa ) for the detection of antidodies against newcastle diesease virus ( ndv ) was established by using purified ndv and self - made enzyme - labeled anti - chicken igg, the mehod was also evaluated through it ' s application

    本研究用提純的新城疫病毒和自製的酶標抗體建立了檢測雞新城疫抗體的dot - elisa方法。試驗中用f _ （ 48 ） e _ 9株和lasota株提純抗原並對兩者進行了比較。
  12. And further studied e rosettes experiment and lymphocyte proliferation brdu - elisa assay

    並對初步篩選的e玫瑰花環實驗和淋巴細胞增殖brdu - elisa實驗進行了進一步篩選。
  13. Finally, we ensure immunobiologic activity evaluation methods of gpif wre t cells from pigs thymus of e rosettes experiment and pha inducing lymphocyte proliferation brdu - elisa assay

    最終確定gpif免疫生物活性評價方法為： t細胞來源於胸腺的e玫瑰花環實驗和pha誘導的淋巴細胞增殖brdu - elisa實驗。
  14. 3. the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme, the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona. the results showed that lymphocyte proliferation induced by pha was better than by cona. 4

    3 、 gpif免疫生物活性評價方法的優化對gpife玫瑰花環實驗在不影響srbc活性的基礎上用神經氨酸酶處理，經此法處理后e玫瑰花環形成率明顯提高；對傳統的cona誘導的人外周血淋巴細胞增殖brdu - elisa實驗，進行了方法學改進。
  15. 2. the comparison of methods of immunobiologic activity evaluation : in this paper, we primitivily selected two kinds of morphologic methods to evaluate gpif immunobiologic activity : e rosettes experiment, lymphocyte transformation experiment, and two kinds of optic colorimetry immunobiologic activity evaluation methods : cell proliferation mtt assay, cell proliferation brdu - elisa assay

    2 、 gpif免疫生物活性評價方法的比較篩選初步比較篩選了兩種形態學免疫生物活性評價方法， e玫瑰花環實驗和淋巴細胞轉化實驗及兩種光學比色免疫生物活性評價方法，淋巴細胞增殖實驗mtt法和淋巴細胞增殖實驗brdu - elisa法。
  16. Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution

    該純化cpe對小白鼠的半數致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。
  17. Sandwich elisa assay is a sensitive, specific and stable technique

    鞏乃a法檢測可溶性比卜工靈敏、特異、穩定； 2
  18. Methods : sandwich elisa assay was used, w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody. the second antibody - hrp conjugate was added for coloration. standard curve was obtained by shla - i standard reagent in serial dilution. the amount of shla - i in the samples was determined : 1

    方法：以w6 32包被酶標板，捕捉樣品中可溶性hla ，加入一抗2m抗體，再加酶標二抗及底物顯色。根據可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線： 1
  19. [ methods ] consideration of free heparin, unlike polypeptides, adsorb poorly onto plastic surfaces. in order to study the interaction of heparin with ifn - y using elisa assay, heparin - bovine complex ( hbc ) was synthesized by conjugating heparin to bsa in the presence of sodium cyanoborohydride

    結果凝膠過濾層析獲得了高純度的hbc ，通過hbc將肝素包被在酶標板上， ellsa實驗表明， ifn一y主要與hbc中的肝素結合（ p （ o ， 01 ） 。
  20. The expression activity reached the highest point at the 72nd hour in bmn cell ( 22 u / 2x ! 06cells ) and at 144th hour in larvae ( 159 u / ml ), respectively. elisa assay showed that expression product had angiostatin ' s immunoreactivity. western blotting assay also showed that product expressed in cultured cells was a 36 kd band, while product expressed in larvae of silkworms was two proteins, and the molecular weights were 37 kd and 42 kd, respectively

    表達產物活性在家蠶細胞中表達72小時達到最高值，經胞內表達產物作用后，血管內皮細胞的存活率僅為（ 28 . 0 3 . 0 ） ，表達量約為22u 2 10 ~ 6個細胞；在家蠶體內表達144小時活性達到最高值，內皮細胞的存活率僅為（ 6 . 4 0 . 5 ） ，表達量約159u ml 。
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