embryo fibroblast 中文意思是什麼

embryo fibroblast 解釋
胚胎成纖維細胞
  • embryo : n (pl embryos)1 【植物;植物學】胚;【動物;動物學】胚胎(對人來說,一般指三個月以內的胚胎)。2...
  • fibroblast : n. 【生物學】纖維原細胞。adj. -blastic
  1. From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain

    本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。
  2. Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection

    亞群禽白血病病毒的雞胚成纖維細胞系建立
  3. These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses

    以上結果顯示, 6 dmap能增加胚胎幹細胞異種重構卵的卵裂率,對重構卵囊胚的發育率影響不大;高培養代數和低培養代數的胚胎幹細胞用於異種核移植不影響異種重構卵的卵裂率和囊胚發育率;用胚胎幹細胞作為供核細胞;比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程,並且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。
  4. Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast

    66弱毒株在雞胚成纖維細胞上的培養
  5. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ;將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點,通過酶切鑒定獲得了pfgs11ha ;將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ,再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平,后再共同用sac酶切連接得puc18pelf , sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點,通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  6. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。
  7. From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken

    為確定該病的病原,對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定,證明該分離病毒與細小病毒科( parvovirdae )細小病毒屬( parvovirus )的雞細小病毒( chickenparvovirus )特性基本相符,核酸型為dna型。
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