embryo mouse 中文意思是什麼
embryo mouse
解釋
胚鼠-
Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed
摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。 -
These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses
以上結果顯示， 6 dmap能增加胚胎幹細胞異種重構卵的卵裂率，對重構卵囊胚的發育率影響不大；高培養代數和低培養代數的胚胎幹細胞用於異種核移植不影響異種重構卵的卵裂率和囊胚發育率；用胚胎幹細胞作為供核細胞；比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程，並且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。 -
The aim of this study was to examine ppar5 expression in rat and mouse uterus during early pregnancy, pseudopregnancy, delayed implanation, artificial decidualization and regulation by steroid hormone treatment by in situ hybridization and inununohistochemisny the expression of ppar gene in preimplanation embryo was also determined by rt - pcr
本實驗以大鼠和小鼠為材料，利用原位雜交和免疫組化方法檢測了ppar基因在早期妊娠子宮中的表達，並利用假孕、延遲著床、人工蛻膜化及激素處理等模型研究ppar基因在子宮中的表達與調節。 -
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
In order to improve the cuture system of mammal embryo and advance the quality of embryo in vitro, the effects of taurine and ethylenediaminetetraacetic acid ( edta ), estrogen ( e2 ) and progesterone ( p ) on preimpiantation development of mouse embryos in vitro were examined and the quality difference of blastocysts from in vitro and in vivo by blastocyst staining and embryo transfer was evaluated
為完善體外培養體系，提高體外發育的胚胎質量，本實驗研究了牛磺酸（ tau ） 、 edta 、雌激素（ e2 ）和孕激素（ p ）對昆明白小鼠胚胎體外發育的影響。在此基礎上，通過胚胎移植技術觀察了不同來源的囊胚在移入子宮后的發育情況，以比較體外培養和體內發育的囊胚質量。 -
In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。 -
To select the best mouse blastocysts resulting from mm16, mm16 + e2, mm16 + p and mm16 + e2 + p mediums, and from ivf - ivc ( in vitro fertilization and in vitro culture ) in htf medium, and from in vivo was for embryo transfer
分別選取小鼠1 -細胞胚胎在mm16 、 mm16 + e2 、 mm16 + p 、 mm16 + e2 + p各組培養液中培養的囊胚、小鼠體外受精-體外培養的囊胚和體內正常發育的囊胚，進行子宮內移植。 -
We also investigated the pathological changes of mouse liver, thymus and cerebrum cortex challenged by so2 inhalation by in vivo tests. we studied the apoptotic induction on mouse spleen cells and cytotoxicity of human embryo lung fibroblasts of so2 derivatives by in vitro tests. in vivo tests of sulfur dioxide inhalation showed : ( 1 ) effects on mouse lung of so2 challenge : we found no significant apoptotic changes induced by so2 inhalation but obvious pathological changes of lung with vacuolating of osmiophilic multilamellar bodies which maybe related with the decrease of surfacant and decrease of microvillus of type ii alveolar cells ; we also found thickening of part of basement lamina between type i alveolar cells and capillary endothelium cells which may inhibit the dispersion of oxygen and contribute to lung dysfunction
二氧化硫熏氣染毒的體內實驗結果表明，在本次實驗的濃度范圍內（ 56mg m ~ 3 、 112mg m ~ 3 、 168mg m ~ 3低、中、高三個濃度） ： （ 1 ）通過透射電鏡、 dna凝膠電泳分析和流式細胞分析發現二氧化硫吸入染毒一周對小鼠肺臟沒有明顯的凋亡誘導作用，但通過透射電鏡觀察發現二氧化硫可引起肺臟明顯的超微結構改變，引起型肺泡上皮細胞板層體空泡化，微絨毛減少，線粒體緻密化或腫脹變性；肺泡血管內皮細胞和型肺泡上皮細胞之間基膜增厚，使氧氣彌散功能出現障礙，從而降低肺功能。 -
It seemed that no was involved in early mouse embryo development
這表明n0參與了小鼠的早期胚胎發育。 -
Marie - therese. loones, sandra m. have investigated the developmental expression of heat shock proteins in the nervous system of the unstressed mouse embryo. the results suggested the biological importance of hsps in neuronal differentiation and migration, as well as in cell signaling, protein transport
Marie - therese . loones ， sandram . dsouza分別研究了熱休克蛋白在小鼠胚胎期和出生后的神經系統發育過程中的分佈情況，推測hsps在神經發育過程中可能與神經細胞的分化、遷移以及神經細胞內的信號轉導和蛋白質的運輸有關。 -
The expression of rhoa in uterine endometrium during the period of peri - implantation of mouse embryo
在小鼠胚胎圍著床期子宮內膜的表達及其意義 -
In conclusion, so2 can not only affect respiratory system, but also other organs such as spleen, liver, thymus on their histological structures and every organ performs very differently under so2 exposure. so2 derivatives has cytotoxicity both on mouse spleen cells and human embryo lung fibroblasts
說明二氧化硫代謝衍生物對人胚肺成纖維細胞和小鼠脾細胞都有一定毒性，並且在一定范圍內對小鼠脾細胞具有一定的凋亡誘導作用。 -
A significant level of 3galt - 1 transcript was present in the brains of 18 - day - old mouse embryo, newborn and 1 - week - old mice. hereafter, its level decreased markedly. densitometry analysis indicated that the p3galt - l band was 10 - fold more intensive in the 1 - week - old mouse brain than that in the 12 - week - old, indicating that 3galt - l mrna was expressed at a developmentally regulated way
我們用northernblot的分析方法發現在18天齡胚鼠、新生鼠和1周齡鼠的腦組織中3galt - 1表達很高，隨著發育進程，它的表達水平明顯降低：灰度掃描定量分析表明1周齡鼠腦中3galt - 1的表達量大約是12周齡鼠腦表達水平的10倍，這說明3galt - 1的表達是受發育調控的。 -
The objective of this study was whether the reconstituted embryo with the second polar body ( pb2 ) can develop. firstly, female mouse was superovulated with pmsg + hcg and keep in the same cage with male mouse, the pronuclear embryo was obtained at the different time after hcg injection with different motheds to investigate the existing time of pronuclear embryo. then pronuclear embryo was cultured with three different mediums to select the best to culture in vitro
母鼠在注射hcg后與公鼠同籠交配，並在注射hcg后不同時間（從18h到26h ） 、不同方法（體內法和體外法）獲取胚胎，研究獲取原核胚的最佳時間；在此基礎上獲得具有第二極體原核胚，然後對原核胚進行顯微操作，去除雌原核並注入第二極體，探討第二極體內遺傳物質支持胚胎發育的潛力；同時利用dna特異性染料h33342對原核胚進行染色，顯示並比較原核和第二極體內的dna遺傳物質。 -
The results demonstrated that the expression of grp78 and grp94 was in a time specific pattern. high levels of grp78 were present in early embryo stage, and were decreased during the fetal period, increased gradually during postnatal development, and reached adult mouse after one week. but the expression of grp94 was initially observed in low levels, and raised gradually along with the development process until the later stage
結果1 .小鼠腦發育過程中分子伴侶g刊階8 、 g刊四4的表達呈現出時間順序性，而且二者的表達模式不同: gr即8胚胎早期表達較高，胚胎發育末期逐漸下降，出生后逐漸升高，至生后一周時達到成年鼠的水平，而『 gr即4在發育的早期表達水平較低，發育中期逐漸升高，出生時達到高峰，出生后一直維持在較高的水平。 -
With a large number of mouse lines of biology engineer are produced, establishment of embryo cryopreservation technology for preservating these resources is very important
摘要隨著生物工程小鼠的大量出現，建立一種完善的胚胎冷凍保存技術體系保存這些資源至關重要。 -
Effect of electrical and chemical activation on reconstructed embryo formed by rat - mouse inter - species somatic cell nuclear transplantation
小鼠種間體細胞核移植重組胚發育的影響 -
Effect of insulin on in vitro development of mouse preimplantation embryo
胰島素對小鼠早期胚胎體外發育的影響 -
In addition, it may also be used as an ideal source of tissue engineering and model of pharmaceutical research and clinical medicine. this study mainly focused on isolating and culturing es cells from mouse embryo and pgcs from human counterpart
本研究以小鼠和流產胚胎為材料，探討飼養層，細胞因子及其添加物等因素對分離和培養icm來源的小鼠和pgcs來源人類多能幹細胞的影響。 -
These studies demonstrates that not only we could make transgenic mouse embryo with high efficiency by the method of icsi, but also the fresh sperm from cauda epididymis or freeze - thawed dead sperm pre - mixed with exogenous dna had the full development potential to develop into term
本實驗充分表明， icsi精子載體法可以比較高地製造小鼠轉基因胚胎，小鼠附睪新鮮精子及無抗凍劑保護冷凍致死精子都具有足夠的全程發育潛力。
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