embryo-fetal 中文意思是什麼
embryo-fetal
解釋
胚胎-胎兒-
In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。 -
The results demonstrated that the expression of grp78 and grp94 was in a time specific pattern. high levels of grp78 were present in early embryo stage, and were decreased during the fetal period, increased gradually during postnatal development, and reached adult mouse after one week. but the expression of grp94 was initially observed in low levels, and raised gradually along with the development process until the later stage
結果1 .小鼠腦發育過程中分子伴侶g刊階8 、 g刊四4的表達呈現出時間順序性，而且二者的表達模式不同: gr即8胚胎早期表達較高，胚胎發育末期逐漸下降，出生后逐漸升高，至生后一周時達到成年鼠的水平，而『 gr即4在發育的早期表達水平較低，發育中期逐漸升高，出生時達到高峰，出生后一直維持在較高的水平。 -
Taken together, these results indicated that an understanding of how in vitro oocyte maturation, in vitro fertilization and embryo culture systems influence both preimpiantation embryo and fetal development should result in systems that consistently produce normal embryos and fetuses
由此說明，體外受精的胚胎的質量顯著低於體內受精的；體外培養液中同時添加雌激素和孕激素能協同促進小鼠胚胎的體外發育，提高胚胎移植后的發育潛力。
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