exchange chromatography 中文意思是什麼

exchange chromatography 解釋
離子交換色層法
  • exchange : vt 1 (以某物與另一物)交換,調換 (for) 2 互換,交流,交易。3 兌換。 vi 1 兌換 (for) 2 交換;...
  • chromatography : n. 【化學】層析,色層(分離)法。
  1. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化,純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時,還呈現抗血小板聚集活性。
  2. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法,從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。
  3. Purification of isomalto - oligosaccharide by cation exchange chromatography

    離子色譜分離法提純異麥芽低聚糖
  4. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌( xerocomusspadiceus )子實體粗提物中,經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾,純化了磚紅絨蓋牛肝菌凝集素。
  5. Conentional methods such as fractionation of complex clinical samples by ionic exchange chromatography and new methods such as enriching low abundant proteins by affinity capture with a combinatorial library of ligands14 proide much needed tools for processing complex biological and clinical samples for proteomics research

    傳統(如對復雜臨床樣品通過離子交換層析進行分離)和現代的方法(如通過親和捕獲與配體庫的組合富集低豐度蛋白)為處理用於蛋白質組學研究的復雜的生物學與臨床樣品提供了極需的工具。
  6. A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc. hwtx - v has 35 amino acid residues, with the molecular mass 4111. 4da. the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh, which consists of 6 cys, formed three pairs of disulfide bridges

    本文報道從虎紋捕鳥蛛( selenocoimahuwena )粗毒中,結合陽離子交換和反相高效液相色譜分離純化出一種昆蟲毒素,命名為虎紋捕鳥蛛毒素- ( huwentoxin - , hwtx - ) ;其天然突變體( themutantofhuwentoxin , mhwtx - )也同時純化出來。
  7. Using i - dopa as a specific substrate, phenoloxidase ( po ) from penaeus chinensis hemolymph was purified by gel - filtration and ion - exchange chromatography, and characterized in terms of its molecular weight and enzymatic properties in this study

    本文以中國對蝦( penaeuschinensis )的血淋巴為材料,利用凝膠過濾和離子交換等方法,對酚氧化酶( phenoloxidase )進行了分離純化和生物化學性質研究。
  8. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵,用緩沖液抽提后,經硫酸按沉澱, deae一纖維素離子交換層析,聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶,用sds一page檢測為一條均一譜帶,其分子量約為78kd 。
  9. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease ( ppase )酶切,酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后,得到純度較高的的m - centrin 。
  10. Sodium tripolyphosphate for industrial use - separation by ion exchange chromatography and determination of the different phosphate forms

    工業三聚磷酸鈉離子交換柱色譜法分離測定不同形式的磷酸鹽
  11. Determination of sulfur amino acids in feedstuffs - ion exchange chromatography

    飼料中含硫氨基酸測定方法-離子交換色譜法
  12. Ion - exchange chromatography a type of chromatography in which the compounds are separated according to their acidity

    離子交換色譜法:是一種根據混合物酸度將其分離的色譜技術。
  13. To reveal the reason of above phenomenon, we purified the eluant by poros 20hq ion - exchange chromatography and got five proteins. one of them ( hq - s ) was proved to can be proteolytic

    為弄清這一現象的產生原因,將嗜硫色譜洗脫峰通過poros20hq離子交換色譜進一步分離,獲得5個分子量非常接近的組分。
  14. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法,從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  15. High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria, which was shown as follows : firstly, physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography. for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography. chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied

    本文利用高效液相離子交換色譜系統研究細菌學,探討了該方法在細菌定性、定量方面的應用,主要包括三個方面:首先,利用離子交換色譜系統表徵細菌生理、生態方面的變化,首次成功地在色譜上區分了短小芽孢桿菌的芽孢及營養體;考察了不同的培養環境對細菌色譜行為的影響及不同生長階段的細菌的色譜行為。
  16. Polyethylene glycol - accompanied ion - exchange chromatography to purify recombinant hepatitis b virus surface antigen

    聚乙二醇伴隨式離子交換層析分離重組乙肝病毒表面抗原
  17. But the whole level of serum titer in combined vaccine group was higher than others. igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose

    對分離到的血清用飽和硫酸銨鹽析法提取igg ,並用deae纖維素離子交換層析法對提取的igg進行純化。
  18. We have sifted 103 medicinal plants, roughly identified 17 plants might contain antifungal proteins. antifungal protein was purified from cassia sophera linn, by extraction, fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ), cation - exchange chromatography of cm - sepharose ff xk 26, the first cation - exchange chromatography of mono s and the second one, followed by gel filtration of superose 12hr

    對茳芒決明進行了抗菌蛋白的分離純化:經粉碎、磷酸緩沖液浸提、硫酸銨沉澱、 cm - sepharoseffxk26陽離子交換層析、兩次monos陽離子交換層析、 superose12hr分子篩層析可得到具抗真菌活性的蛋白。
  19. In this paper, one interactive protein was isolated and purified further by ion - exchange chromatography, whose molecular weight was determined by sds - page to be 24kda under non - reduced conditions and 25kda under reduced conditions

    最後,分離純化作用蛋白中分子量為24kda的蛋白,發現其存在鏈內二硫鍵,二硫鍵斷裂后的分子量為25kda 。
  20. The bioactive strain ' s fermentation product was isolated and purified primarily using methods of solvent extraction, acid - alkali extraction, ab - 8 macropore absorption chromatography, 1 x 007 positive ion exchange chromatography, the result showed the purification product has prominent bioactivity inhibit staphalococcus aureus

    中國熱帶農業科學院、華南熱帶農業大學2003屆碩士研究生採用有機劑抽提法、酸堿抽提法、大孔吸附樹脂柱層析和陽離子交換樹脂柱層析等方法對hsl 306菌株發酵產物進行了初步分離純化。
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