explant 中文意思是什麼

Regeneration of peach plantlet from callus derived from explant

桃離體組織分化再生植株的研究

With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

（ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。

The process of initial culture showed quite difference in various hostas. h. ventricosa and h. " so sweet " were easier, while h. " frances williams " and h. " gold standard " were difficult. using shoot tip ( peak bud ) as explant was proved the effective method for improving survival ratio when hostas would defoliate or have defoliated in autumn or winter rather than in summer

玉簪屬不同種類的組培初代建立存在著一定的差異，如h . ventricosa和h . 『 sosweet 』較容易建立，而h . 『 franceswilliams 』和h . 『 goldstandard 』比較困難；應在冬季玉簪屬植物將要落葉或已落葉后取莖尖（頂芽）進行初代建立，最好避開夏季，來提高初代建立的成活率。

Advances of explant browning in plant tissue culture

植物組織培養中褐變的研究進展

A section of liver explant is provided

切片示切除的肝臟。

Establishment of leaf - explant regeneration system of populus alba p. berolinensis

銀中楊葉片外植體再生體系的建立

The explant tissue culture was compared with the typsin digestion culture

對比了組織塊培養和消化培養兩種培養方法。

Methods to prevent explant and its calli from browning in tissue culture in pinus thunhergii parl

防止黑松外植體及其愈傷組織褐變的方法

Its application way makes the authority and maintance easy. it is easy to control, and easy to explant

其應用方法具有普遍性，且授權維護方便、可控性強、易移植。

Liver transplantation was performed ; the explant weighed 680 grams and showed diffuse micronodularity on sectioning

肝移植手術中見切除的肝重680克，切面呈彌漫小結節狀。

Such a piece of transplanted tissue is called an explant, and its placement on the culture medium is called inoculation

這種被移植的組織稱外植體，將它放置在培養基上稱為接種。

Liver transplantation was performed ; the explant weighed 770 grams and showed dark - green cut surfaces without obvious nodularity

肝移植術中見切除的肝重770克，切面呈暗綠色，未見明顯結節。

The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。

Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。

4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis

通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，並且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。

The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated. the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one. the cocultivation time is 28 days. the experiment shows that : the erniuxin cotyledon regeneration frequency is very high, and that the dongnong901 hypocotyl regeneration frequency is very high

確立了農桿菌介導轉化過程中基因型是影響轉化率的主要因素。通過極差分析，基因型為「二牛心」 ，菌液濃度15倍的，外植體為子葉，感染方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。

The callus was induced and proliferated using young leave as explant, which comes out at early spring. in this paper, the author discussed the induction effect and the proliferation effect by using different kinds of hormone and different physical - chemical factors

本文以山茶初春新生枝條的幼葉為外植體，採用不同的培養基，探討了不同的植物生長調節物質對愈傷組織的誘導和不同的理化因子對愈傷組織的增殖效應。

S. regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials, and ms as basic medium supplying different ba ^ naa and kt concentration, we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3. 0mg / l + naa0. 2 mg / l ). among the three kinds of mustard explants. the cotyledons showed the highest adventitious bud regeneration capacity. and the regeneration capacity of leaf explantswas weaker. and the weakest of hypocotyls

芥菜高頻再生體系的建立以4天左右的葉用和莖用芥菜子葉、下胚軸和25天苗齡的芥菜無菌苗的葉片為外植體，以ms為基本培養基，通過加入不同濃度和不同組合的ba 、 naa 、 kt ，篩選到了外植體不定芽分化的最適培養基（ ms + ba3 . 0mg / l + naa0 . 1mg / l ）及生根的最適培養基（ ms + naa0 . 2mg / l ） 。

Addition of img / l tdz and img / l naa resulted in the highest bud regeneration rate ( 67. 9 % ) and number ( 35 ) of buds per explant. regeneration rate could be improved apparently in the medium with agno3

1mg ltdz和1mg lnaa配合對子葉再生具有極強的誘導作用，再生率最高，達到67 . 9 ，平均每外植體不定芽數達35個。

( 3 ) the optimal explant age for transformation was 6 or 7 day after aseptic germination of seeds. ( 4 ) development of non - transformated cell was inhibited completely by 100mg / l kan. ( 5 ) 500mg / l cb could avoid pollution of agrobacterium tumefaciens and had no distinct effects on the formation of callus and shoot regeneration. ( 6 ) the optimal co - cultural condition was 3 day on 28 in dark

以胡蘿卜品種「改良黑田」為受體材料，以胡蘿卜幼苗的下胚軸為外植體，建立了農桿菌介導法的胡蘿卜轉化體系，並對其中的種子消毒、激素水平、外植體的苗齡、選擇壓、共培養時間等關鍵步驟的條件進行了優化。