expressed-sequence tags 中文意思是什麼
expressed-sequence tags
解釋
表達的序列標記片段-
Bioinformatics methods are used to analyze and manage expressed sequence tags ( ests ) which are generated by large - scale sequencing of a cdna library from cephalothorax of a wild, female, adult chinese shrimp ( fenneropenaeus chinensis )
本文運用生物信息學的方法，分析及管理由雌性成年中國對蝦頭胸部cdna文庫經部分測序產生的10446條ests 。 -
We have identified a nucleotide sequence from ests ( expressed sequence tags ) acquired form a cdna library of thellungiella halophila treated with 200mm / nacl by the large - scale partial sequencing of randomly selected cdna clones
通過對小鹽芥的cdna文庫大量測序，在1000個est序列中獲得14個編碼th - nsltp的序列，這表明它是一個中等豐度的基因。 -
In addition, more than 30 differential expressed sequence tags ( est ) have been isolated by mrna differential display
我們還利用mrna差異顯示技術分離到了近30個在mnng處理后表達改變的表達序列標簽（ expressedsequenceag ， est ） 。 -
By sequencing random - selected clones from the library and using bioinformatics, 54 novel expressed sequence tags ( ests ) were obtained, in which there were 14 full - length cdnas, including peroxiredoxin gene, hmg - box gene, ubiquitin gene, signal recognition particle, acetyltransferase gene and lysozyme gene
有14個克隆包含有完整的開放閱讀框，其中包括抗氧化物酶基因prx 、 dna結合蛋白基因、泛素基因、信號識別蛋白基因、溶菌酶基因和乙酰基轉移酶基因等具有重要生物學功能的基因。 -
Gene ( tscoxi ), structure protein gene ( tsdcn, tsmmip, tsb - actiri ), transcriptional factor gene ( tshmg1, tsdap5 ). the cdna library can be used to provide expressed sequence tags ( ests ). the stage - specific cdna library will be a useful resource for the study of gene structure, expression and regulatory during the early process of embroygensis of trionyx sinensis
Blast分析表明， 12個序列代表5種類型的基因:核糖體蛋白基因（ tsrpl4 ， tsrp丈6 ，招天屍乙26 ） 、組織特異性表達的基因（鉆屍乃從招月叨火d ） 、線粒體基因（ tsc 「叨、結構蛋白基因伽dc從括人從石， ， ts刀一ctin ） 、編碼轉錄因子的基因（招月沏吟1 ， tsda屍5 ） 。 -
Previously, we have constructed a 400mmol / l nacl - treated cdna library of s. salsa and acquired 1000 ests ( expressed sequence tags ) by the large - scale partial sequencing of randomly selected cdna clones. in this research, we isolated a cdna that maybe encode an aquaporin ( s. salsa plasma membrane intrinsic protein, sspip, bf023789 ) located in plasma membrane of s. salsa. then we analyzed the sequence characterizations, genomic structures and transcriptional levels under salinity stress
本實驗室構建了400mmol lnacl處理的鹽地堿蓬（ s . salsa ）地上組織的cdna文庫，隨機挑取單克隆進行測序，獲得了可能編碼鹽地堿蓬質膜水通道蛋白（ s . salsaplasmamembraneintrinsicprotein ， sspip ）的cdna ，其序列號為bf023789 。 -
In summary, we applied rd - pcr technique to the isolation of cdna fragments, which, compared with the conventional cloning procedure, is more speedy and higher throughput. the rd - pcr cdna fragments were constructed into a s. cerevisiae est ( expressed sequence tags ) library, which lay the foundation of cdna probe preparation and preparation of cdna microarray
綜上所述，本研究採用rd ? pcr技術，探索了利用該技術快速分離大量cdna片段的具體過程，並建立了酵母cdna文庫及酵母cdna探針文庫，為dna晶元的制備打好基礎。
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