extracellular domain 中文意思是什麼

extracellular domain 解釋
胞外域
  • extracellular : adj. 【生物學】細胞外的 （opp. intra-cellular）。
  • domain : n 1 領土，版圖；領地。2 管區，勢力圈；（特定動物等的）生長圈；（學問、活動等的）領域，范圍；【物...
  1. Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome

    為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。
  2. Inspection for the biological activities of recombination protein of ? ctla4 extracellular domain in vitro : we investigated the role of ctla4e in t cell proliferation of mlr with 3h - tdr incorporation

    重組人ctla4胞外區蛋白體內生物學活性研究： l ）觀察ctla4胞外區蛋白在小鼠變應性鼻炎中的作用。
  3. In our study, the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones

    我們將trk受體膜外域1911結構域與gal4蛋白融合構建成pgbkt7一ign ，確認無自激活作用后，將其作為誘餌蛋白，洲于酵母雙雜交篩選。
  4. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  5. Biological assay proved that the expressed product could stimulate the proliferation of cd34 + hematopoietic cells. conclusion : flt - 3 ligand extracellular domain was successfully expressed

    體外活性實驗表明，畢赤氏酵母表達的重組fl蛋白可以有效地刺激造血幹細胞cd34 「的增殖。
  6. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
  7. Screening and constructional analysis of extracellular at the ligand binding domain of the vascular endothelial growth factor receptor flt

    胞外配體結合域的篩選及其結構分析
  8. It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein

    跨膜區域（ tmd ）將它分為胞內和胞外兩個部分，它的胞外有13個潛在的糖基化位點， 20個蛋白激酶c的磷酸化位點， 28個酪蛋白激酶的磷酸化位點， 4個酪氨酸酶的磷酸化位點， 15個豆蔻（十四烷基）酰化位點；它的胞內有1個整合蛋白（ integrin ）識別位點。
  9. Hence, yeast gs115 was used to express the target protein in this study. objection : to obtain the biological activated recombianated protein of huaman ctla4 extracellular domain ( ctla4e ), and to explore its effects on allergic diseases and transplant rejection

    目的：獲取具有生物學活性的重組人ctla4胞外區蛋白（ ctla4e ） ，探討其在變應性疾病和器官移植排斥反應防治中的作用和機理。
  10. 2. the functional of the purified trka extracellular domain proteins were tested using pc - 12 cells

    2 、用pc12細胞株測定腸ka膜外域各結構域重組蛋白的功能。
  11. We found that insulin - degrading enzyme ( ide ) interacts with ge through its extracellular domain

    我們發現胰島素降解酶（ ide ）可通過其細胞外區域與糖蛋白e相互作用。
  12. A leucine zipper pattern is located in the membrane proximal extracellular domain ( mped ) of bt - r3 protein. this is a very important character of cadherin

    另外，它還有1個亮氨酸拉鏈序列，位於胞外的近膜區（ mpr ） ，這是鈣粘蛋白的重要特徵之一。
  13. Cloning of extracellular domain fragment of human light gene and expression of fusion protein

    胞外區基因的克隆及融合蛋白的表達
  14. Meanwhile, because the fragment of ctla4 extracellular domain is only 400bp, its expression will be more stable than that of ctlaig fusion protein ( 1. 2kb )

    同時，由於ctla4胞外區片段大小僅有400hp ，較ctla4ig融合蛋白zkb ）在表達菌中的表達將更為穩定。
  15. Trka, the high affinity ngf receptor, contains an intracellular domain with tyrosine kinase activity and an extracellular domain with three tandem leucine - rich motifs flanked by two cysteine clusters in their amino termini and two immunoglobulin - like domains in the more membrane - proximal region

    膜外域含有8個亮氨酸的重復區域，其前後分別串聯著一組半胱氨酸殘基，緊挨細胞膜的是兩個c2 -型免疫球蛋白樣區ig和ig ；膜內域為酪氨酸激酶催化活性區。
  16. Huctla4 - lg, " one of the most potent in1n1unosuppressive molecules, is a soluble recombinant fusion protein ( molecu1ar weight of approximately 92 kd ) consisting of the extracellular domain of human ctla - 4 and a fragment ( hinge and constant region ) of the fc portion of human iggl. it strongly adheres to the b7 molecule to block the cd28 - mediated costimulatory signal, resulting in inhibition of in vitro and in vivo immune response

    可溶性重組融合蛋白huctla4 - ig是一種新穎的免疫抑制劑，分子量約為92kd ，由人ctla - 4分子的胞膜外區與人igg1的fc段（鉸鏈區與恆定區）融合形成，能牢固地與b7分子結合，阻斷cd28介導的共刺激信號，導致免疫反應的抑制。
  17. [ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t

    L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。
  18. Considering that aspartic acid ( d ) and glutamic acid ( e ) are very similar to each other, together with the biological function of the landmark, thus that the two landmarks can be regarded as no difference ; 3 ) conservative cysteine in terms of their numbers and positions in the extracellular domain. in ghr1 group, fghrl and zfghrl have seven cysteins, which are the same as that of quadruped. in ghr2 group which includes cohoghr isf1, cohoghr isf2, chghr, fghr2 and zfghr2, the cysteine number and positon are very similar to each other but are different from ghr1 group. especially, the fghr2 has five cysteins that is one more than zfghr2 ; 3 ) conservative boxl domain. with all the ghrs identified so far, boxl are comprised of ppxpxp ( x represents any amino acid )

    在fghri和zfghri都為7個，數目和四足動物ghr一致;在fghrz胞外膚氨酸殘基為5個， zfghrz為4個，和同屬于ghrz的eohoghrisfl 、 eohoghrisfz 、 ehghr基本保持一致，但是卻和傳統的ghri有比較大的區別; 3 ）本試驗克隆和分離得到ghri和ghrz以及所有目前發表的ghr都有比較保守的boxl結構域，氨基酸組成為ppxpxp ，研究表明最後兩個脯氨酸殘基作用最大; 4 ）在所有ghr中都完全一致的boxz ，序列為料efi ; 5 ）在ghri和ghrz胞內部分都比較保守的酪氨酸殘基，他們被認為在信號傳導中起作用。
  19. Methods : 1. expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique. then, this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ). furthermore, the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed

    重組人ctla4胞外區蛋白在酵母gs115中的表達和純化：首先通過基因操作技術，從pctla4八g質粒中擴增400hp的ctla4胞外區片段；將ctla4胞外區片段插人ppicg質粒中獲取單拷貝酵母表達質粒ppicg ctla4125並進一步構建多拷貝酵母表達質粒ppicgk （ 。
  20. Ctla4ig fusion protein was expressed by genetically fusing the dna fragment encoding 1 - 125 amino acid residues of ctla4 extracellular domain with dna fragment encoding an igg fc region. its in functional region is the extracellular domain of ctla4

    Ctla4ig融合蛋白是通過基因重組技術將編碼ctla4胞外區1 - 125個氨基酸序列的dna片段與編碼iggfc段的dna片段融合后表達生成，其功能區是ctla4胞外區。
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