feeder cell 中文意思是什麼

feeder cell 解釋
飼養細胞,喂養細胞
  • feeder : n 1 給食者；餵食者；供應者；飼養員；煽動者，慫恿者，填鴨式教人的人。2 寄食者，使喚人；食客。3 奶...
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. Es - d3 cells differentiated into ebs only when depleted of feeder cell layer by being cultured three generations in the presence of leukemia - inhibitory factor ( lif )

    結果表明： eso3細胞在含有lif的培養液中脫離飼養層培養3代以上才能形成ebs 。
  2. There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation. es - like cell develop embryoid body - like structure in suspension culture in vitro. es - like cells of goat differentiate into various kinds cell, egg : fibroblast cell, neurolike cell

    Es細胞在體外懸浮培養，形成類胚體，山羊類es細胞在無飼養層鋪有明膠的皿底上培養，可分化為多種類型細胞，如上皮細胞、成纖維細胞、神經細胞等。
  3. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  4. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium

    聯合evans和martin的方法，稍加改良來分離小鼠胚胎幹細胞，把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上，在含有brl條件培養基的es細胞培養液中培養。
  5. The device constructed a hopper, as a buffer tank, a screw feeder, a weighing - cell, a fill plate, a motor drive, and a load - cell based weighing controller

    主要的構件有進料漏斗、螺旋送料器、荷重元、盛料斗、驅動馬達及重量指示器等。
  6. With the increase of passage, the total of colonies decreased, but the number of classical nest - like or island - like eg cell colonies relatively increased. the growth of eg cells was dependent on the feeder layer

    傳代培養的eg細胞對飼養層有依賴性， icr品系小鼠pgc細胞和由其傳代后獲得的eg細胞呈明顯的群聚性，以單個存在狀態的較少
  7. We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines

    以icr小鼠和昆明小鼠為實驗對象，採用超數排卵的方法獲得小鼠胚胎，培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上，選取適當時間離散增殖的icm和類es細胞集落，分析了在小鼠es細胞建系過程中，飼養層細胞、消化液及不同品系來源的胚胎的影響。
  8. ( 4 ) the myocardium cell group on embryonic body fibroblast feeder was cultured successfully. the myocardium tissue grew gradually, its contraction changed with temperature and morphology changed with time, and not all myocardium tissue could contract

    （ 4 ）小鼠胚體成纖維細胞可以與心肌細胞團共培養，並出現自律性收縮，細胞團逐漸增長，其收縮情況隨溫度變化而變化，隨時間變化其形態學發生變化，而且並非所有心肌細胞團都能收縮。
  9. After 4 days of growth, the intact inner cell mass ( icm ) were separated with 0. 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum, 0. 1mmol / l nonessential amino acids, 0. 1mol / l 2 - mercaptoethanol, 2mmol / l glutamine, 100 units / ml of streptomycin and 100 units / ml of penicillin. after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected

    （ 2 ）小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚，經過3 ? 4天培養后，將增殖出來內細胞團用機械法結合胰酶- edta處理，離散后培養于mef飼養層上， 4 6天後有5例出現了es細胞樣集落。
  10. The enhancing factors of eb formation were studied. fifty thousand es - d3 cells per milliliter were incubated in dmem - high glucose supplemented with 20 % fbs and 2mm glutamine. the influencing factors which included hyclone fbs and homemade green season fbs, feeder cell layer, culture containers and 3 - mercaptoethanol ( - me ) were investigated

    以5x10 』加的eso3細胞種入含20胎牛血清和zinm谷氨酚胺的高糖dmem中，觀察進口胎牛血清和國產胎牛血清、飼養層、培養器皿以及卜硫基乙醇對es士3細胞形成的4debs數量的影響。
  11. The blastocyst was obtain from the mouse after 3. 5 days of pregnancy, and was cultivated on the mouse embryonic flbroblast feeder layer. the blastocyst usually attach to the feeder layer after 48 hours, then the inner cell mass began to grow and form a big cell mass of embryonic stem cells. these cells can form cell clones with the conformation of embryonic stem cell

    囊胚一般在48小時后貼壁並脫去透明帶，由囊胚中的內細胞團增殖形成一個胚胎幹細胞球，待其生長至一定程度后對其進行酶解傳代，在傳代后的細胞中重新生成es集落形態的細胞集落，在集落生長至一定程度后再進行傳代，此後大約每5 ? 7天傳代一次。
  12. After being cultured three generations and depleted feeder cell layers, the es - d3 cells differentiated into ebs efficiently in the culture media supplemented with homemade green season fbs and - me in petriperm bacterial - culture dish

    二、 inbmec cm誘導小鼠es細胞生成造血干祖細胞將獲得的4debs製成單細胞懸液，加入inbmec cm誘導4debs的單細胞生成造血干祖細胞。
  13. Two pgcs were cloned to forth passage supplied with lif and scf. pgcs were cloned to third passage supplied with lif and scf on feeder cell. pgcs has the characterization of es - like

    採用飼養層培養法，在培養基中添加lif和scf ，分離到了原生生殖細胞，並有2個pgcs集落傳至第3代。
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