fetal bovine serum 中文意思是什麼
fetal bovine serum
解釋
胎牛血清-
The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells
血清濃度的高低對胎鼠成纖維細胞和es細胞的培養過程是有影響的。無血清或低濃度的血清能抑制細胞分裂增殖，使細胞生長出現停滯。 -
In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks
方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。 -
Induction of neural stem cells from newborn fetal guinea pig by fetal bovine serum and artificial perilymph
新生豚鼠海馬神經幹細胞分化為類毛細胞的體外實驗 -
The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。 -
Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum
含胎牛血清膠原酶消化法培養兔成骨細胞 -
The culture results of the porcine ear skin fibroblasts was good by using dmem / f12 containing 20 % fetal bovine serum
在培養條件方面選取dmem f12培養液+ 20胎牛血清培養豬耳皮膚成纖維細胞，效果較好。 -
We can see a lot of new pictures of induced neuron - like cells, include neuron - like cells and glial - like cells, with the apparent nerve cells. material and methods rat bone marrow cell were collected, after sacrifice of a 3 months old wistar rat, from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml
無菌條件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培養液1000rpm離心10分鐘，吹打b人兩個培養瓶，置人37 j coh飽和濕度條件下培養，隔日更換培養液，當細胞達到70融合時，傳代。 -
The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively
主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
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