flanking dna 中文意思是什麼
flanking dna
解釋
側翼dna-
Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion, and high - level dna amplification of those sequences flanking the deletion
許多鏈黴菌表現出高度的遺傳不穩定性，通常為大片段缺失或基因組重排，以及缺失區域兩側序列的高水平dna擴增。 -
The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr, and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5為標簽，用錨定pcr法克隆出突變株nm4的tn5側翼序列。並以此側翼序列為標簽繼續用錨定pcr法向兩邊延伸，克隆出一個5045bp的dna片段。 -
With h. trueperi genomic dna and degenerate primers, a 560 bp pcr fragment was obtained and labeled as a probe. after h. trueperi genomic dna was digested with different endonucleases, southern blot result showed a 2. 6 kb positive fragment digested by ecori and ipcr was carried out to obtain the flanking sequence
將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southem雜交，結果顯示在ecori酶切片段的2 . 6kb處有陽性信號。 -
Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1
通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。 -
Dna sequences flanking tn5 - 1063 of 042bmr5 were amplified using inverse pcr, it was found that tn5 - 1063 was inserted into noeb gene
對042bmr5突變株的基因組進行反向pcr ，擴增位於tn5 - 1063兩端的側翼序列。
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