flanking sequence 中文意思是什麼
flanking sequence
解釋
側面序列-
( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
（ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。 -
The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences
以lacz為報告基因的瞬間表達實驗結果表明，長度分別為306bp和302bp的crtw和crtz5 '上游側翼序列具有很強的啟動轉錄活性，提示兩段序列包含了啟動子的結構。 -
In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。 -
We amplified aroa, aroc, arob of common pathway, csra and its flanking sequence of global regulation network from e. colt, and kan resistant gene from plasmid pet28a. 4. centerpiece of this
在bioflo3000bateh / continuousbioreaetor （ sl ）進行發酵試驗結果證實，發酵液中副生雜酸較少，產品經初步純化后純度較高，可達93 % 。 -
The main difficulty in development of microsatellite ( or simple sequence repeats, ssr ) is to screen the specific sequences flanking ssr, a method to screen microsatellite based on sampl was tested in the chinese shrimp. randomly selective bands ( s1k s13 > s14 > s22 > s24 ) were cloned and sequenced, 61bp, i57bp 147bp, 152bp, 298bp sequences were obtained respectively. no ssr were found inslk s s s22 apart from the sampl primers sequence, on the other hand, a ssr sequence of ( ga ) 39 were found in s24, which comprises aflp primers only
微衛星的發展主要受限於微衛星兩端旁側特異引物的篩選，本研究在中國對蝦中初步嘗試了基於sampl技術的微衛星標記開發，隨機選取了5個s枷pl條帶（ 511 、 513 、 514 、 522 、 524 ）進行了克隆測序，大小分別為161bp 、 157bp 、 147bp 、 152bp 、 298bp ，但在511 、 513 、 514 、 522四個片段中未找到引物序列外的微衛星序列重復; 524兩端引物均為傳統的aflp引物，在此測序片段中有一（ ga ） 39重復序列。 -
In this study, using bioscien tomato as test material and the flanking region of the bioscien insert sequence was determined using inverse pcr and real - time pcr. by means of verification, this sequence is specific for the transfection event
本研究以華番1號為試材，利用反向pcr和熒光pcr技術已成功將華番1號轉基因整合位點dna序列擴增出來。 -
Gene promoter on its 5 flanking sequence
基因5末端側翼序列啟動子的分析 -
Gene promoter on its 5 flanking sequence analysis of the mouse
基因5末端側翼序列啟動子的分析 -
With h. trueperi genomic dna and degenerate primers, a 560 bp pcr fragment was obtained and labeled as a probe. after h. trueperi genomic dna was digested with different endonucleases, southern blot result showed a 2. 6 kb positive fragment digested by ecori and ipcr was carried out to obtain the flanking sequence
將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southem雜交，結果顯示在ecori酶切片段的2 . 6kb處有陽性信號。 -
Two crtw 5 ' - flanking upstream sequences ( 795bp and 695bp, respectively ) and one crtz 5 ' - flanking upstream sequence ( 302bp ) are obtained using ada ptor - primer pcr method
分離到兩個crtw5 '上游序列和一個crtz上游序列，長度分別為795bp 、 695bp和302bp 。 -
Study on amplification of blg 5 - flanking and regulatory sequence by pcr
擴增的方法學研究
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