fluorescence microscopy 中文意思是什麼
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Mycobacteria can also be stained with auramine and viewed with fluorescence microscopy, in which acid fast bacilli now appear as glowing yellow rods
分枝桿菌也能被金胺染色，熒光顯微鏡下抗酸桿菌為發黃*色熒光的桿菌。 -
Laser confocal fluorescence microscopy
激光共聚焦熒光顯微鏡 -
Confocal microscopy observation followed immune - fluorescence staining with anti - gp130 showed that gp130 could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus
為了進一步證實上述發現，我們表達並純化了gst - gp130胞漿區融合蛋白和gst - tle1獨特性片段融合蛋白，並制備了特異性抗tle1多克隆抗體。 -
We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast
本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。 -
On the backgrounds of researches inside and outside country, and cooperating experiments with theories analyses, the influence of different processing technology parameters and different sbs modifier sorts on the sbs modified asphalts " properties has been studied. at the same time, their microstructure are observed through fluorescence optical microscopy and scanning electronic microscopy, thus to direct modified asphalt production. on the above conclusion ' s basement, analysing some disadvantages of the storage stability test of sbs modified asphalt in the current specification, a new storage stability test apparatus is developed
本文在參考國內外研究的基礎上，採用理論、試驗相結合的方法，研究加工工藝參數以及改性劑種類等對sbs改性瀝青性能的影響，並通過熒光顯微鏡、掃描電鏡分析其微觀形態，從而指導sbs改性瀝青的生產；在此基礎上，分析我國現行規范用來評價sbs改性瀝青儲存穩定性方面的不足，開發了新的試驗儀，根據動態剪切流變試驗結果和微觀狀態分析，提出一個新的指標? ?離析率r _ s來評價sbs改性瀝青的儲存穩定性；最後，針對不穩定的改性瀝青提出改善措施，研究證明摻加增容劑和穩定劑是行之有效的方法。 -
This study was focused on the occurrence characteristics of the cryptomelane - bearing ores and the mineralogical characteristics of natural cryptomelane. the morphology, chemical and structure features of natural cryptomelane were characterized by means of powder x - ray diffraction, scanning electron microscopy, electron probe microanalyzer, energy dispersive spectrometer and x - ray fluorescence
利用x -射線粉晶衍射掃描電鏡電子探針電子能譜和x熒光光譜對天然錳鉀礦的形貌特徵化學成分結構特徵進行研究，結果表明天然錳鉀礦晶體形態主要為針狀纖維狀，沿 -
Twophoton laser scanning fluorescence microscopy and its applications
雙光子激光掃描熒光顯微鏡及其應用 -
The composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, and x - ray photoelectron spectra, ellipsometry, scanning electron microscopy, atomic force microscopy, transmission electron microscopy, fluorescence spectroscopy, and standard four - probe technique
採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、橢圓光度法、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、熒光光譜和標準四探針技術對所制備的納米復合膜進行了組成、結構和性能表徵。 -
Fluorescence power transfer function, three - dimensional point spread function ( 3d - psf ) and three - dimensional optical transfer function ( sd - otf ) for the various fluorescent wavelength of the two kinds of fluorescence confocal scanning microscopy are calculated in this paper by using fourier imaging theory. the results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut - off frequency, resolution and 3d - otf. there is a different missing - cone in the 3 - d space of otf when the ratio of excitation wavelength to fluorescent wavelength decreases
本文在sheppard和gumin等人的理論基礎上，利用fourier光學成像理論，討論了不同熒光波長對單光子和雙光子共焦顯微鏡成像特性的影響，導出了單光子和雙光子共焦顯微鏡的熒光功率傳輸函數、三維脈沖響應函數和三維光學傳遞函數，得到了它們在不同激發波長與熒光波長比值時具體的表達式，並且通過數值計算，得到了它們的曲線圖，結果表明：隨著激發波長與熒光波長比值的增加，焦斑的橫向分佈和縱向分佈變窄，橫向解析度和縱向解析度提高，系統的成像效果變好，當激發波長與熒光波長的比值下降到一定程度時，可以看到不同程度的失錐現象。 -
After 12h - long transport, 6 ( 5 ) cf could be detected in the stock by using fluorescence microscopy. the result of transport was accord with the histological observation
證實該時期6 （ 5 ） cf可以自接穗跨過嫁接面向砧木運輸，這與組織學觀察結果一致。 -
Fluorescence microscopy a form of microscopy in which fluorescent probes are added to the material being investigated
熒光顯微術：用來觀察研究插入熒光探針后的物體的一種顯微技術。 -
In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。 -
Biofilm containing srb was observed on the surface of 316l ss by fluorescence microscopy in medicated postgate ' s c medium inoculated srb
熒光顯微鏡觀察發現，在接種srb的修正的postgate ' sc培養基中， srb在316lss表面附著發展並形成生物膜。 -
H2o2 generation in guard cells was examined by laser scanning confocal microscopy based on fluorescence probe h2dcfda. the fluorescence intensity in guard cells of wild type and ios5 was essentially the same before treatment
以h2o2熒光探針h2dcfda結合激光掃描共聚焦顯微技術直接檢測了保衛細胞內的h2o2產生情況。未加任何處理之前，野生型與los5保衛細胞內熒光強度幾乎相等。 -
Furthermore, when 10 imo il h2o2 ( final concentration ) was added to the suspension after above treatment caused little reduction in hpts fluorescence. the results obtained by laser scanning confocal microscopy suggested that addition of exogenous aba resulted in a rapid decrease in fluorescence in most cellular compartments of the guard cells
維生素c可部分逆轉低濃度h2o2 （ 10 - 5 ）所誘導的氣孔關閉過程：並且10 - 6mol l的dpi和103u ml的過氧化氫酶（ cat ）在一定程度上也逆轉了aba誘導張開氣孔的關閉。 -
Based on epidermal strip bioassay, microinjection, patch - clamp and laser scanning confocal microscopy in the experiments, we provided the first evidence that map kinases, including mek1 / 2 or p38 / hog1, plays an important role i n aba - or sa - induced h2o2 signal initial, amplification and specific targeting in response to stimuli in guard cells. aba - or h2o2 - induced vicia faba stomatal closure. was inhibited or reversed by the specific inhibitor pd98059 of mek1 / 2 ; the guard cells were pre - incubated or - microinjected by 10 umol l - 1 pd98059, aba could not enhance the fluorescence intensity of h2o2 probe dichlorofluorescein ( dcf )
在對照實驗中， aba誘導熒光迅速增高；單獨的pd98059 、 pd98059和aba共同處理氣孔時，保衛細胞內h _ 2o _ 2探針熒光強度沒有增高；將pd98059注射進入其中的一個保衛細胞，再以aba處理，使得兩個有同樣熒光基礎的保衛細胞熒光強度對比強烈；將pd98059顯微注射進入已被aba誘導dcf （ dichlorofluorescin ）熒光強度升高的氣孔保衛細胞，熒光強度下降，而沒有被注射一邊的保衛細胞中的dcf熒光強度不變。
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