fluorescence-based dna sequencing 中文意思是什麼
fluorescence-based dna sequencing
解釋
熒光法dna測序- fluorescence : n. 熒光（性）。
- based : 基準
- dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
- sequencing : 測序，序列測定
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That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers, then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting, and deposited by ethanol ). sequencing analysis of pcr product was done on 310 gene scan analysis ?. 1. 2 dna sequencer to determine telomerase activity. notably, this method eliminated the restraining factors of taq dna polymerase, making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results
在kim等開發的端粒重復擴增分析法（ trap ）的基礎上進行改進，即通過對端粒酶延伸ts寡核昔酸反應產物的精製，消除了pcr擴增中抑制taq酶活性的因素，從而減少了樣品之間pcr擴增上的差異和假陰性現象的發生，提高了判斷樣品端粒酶陰、陽性的準確率和定量的準確性。
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