fluorescent staining method 中文意思是什麼
fluorescent staining method
解釋
熒光染色法- fluorescent : adj. 1. 熒光的，發熒光的。2. 外表華麗的；光輝四射的。3. 〈美口〉容光煥發的。n. 〈美口〉熒光燈。
- staining : 斑點染色
- method : n 1 方法，方式；順序。2 （思想、言談上的）條理，規律，秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
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Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
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