foreign dna 中文意思是什麼
foreign dna
解釋
外源-
Because of the special biological structure of bryology, it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation. protoplasts as acceptor, using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way
由於蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化，以原生質體作受體是蘚類植物轉化的常用途徑。 -
Electroporation of sperm to introduce foreign dna into the genome of pinctada maxima jameson
大珠母貝精子介導外源基因轉移研究 -
At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。 -
The chimaeric molecule, containing the vector dna and inserted foreign dna, is introduced into bacterial cells where it multiplies.
含有載體的DNA以及插入的外源基因的雜合子，被引入細菌細胞，並且在其中增殖。 -
The chimaeric molecule, containing the vector dna and inserted foreign dna, is introduced into bacterial cells where it multiplies
含有載體的dna以及插入的外源基因的雜合子，被引入細菌細胞，並且在其中增殖。 -
They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection
用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核，並移植入假孕受體鼠，產生的65隻n代小鼠中，經pcr和southern雜交檢測，表明轉入的基因在12隻小鼠體內有整合段。 -
Because there are many copies of chloroplast dna and the chloroplast has a strong tolerance to accumulation of the products expressed by the introduced foreign gene, a high level of expression is often happened in chloroplast transformation. in addition, because of prokaryotic property of the chloroplast, the prokaryotic gene can be expressed in chloroplast without any modification and multigene can be simultaneously transferred in " polycistron ", which is impossible in nucleic transformation
另外，由於葉綠體基因組的原核性質，對來自原核生物的外源基因無需改造就可以在葉綠體內高效表達，而且可以將多個外源基因採取「多順反子」的原核表達形式同時引入，並由共同的啟動子控制，既方便操作又可避免由於存在多個相同啟動子所帶來的「共沉默」 。 -
An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1. 3.
圖13列出了引出一段外源DNA進入克隆載體的大概步驟。 -
An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1. 3
圖1 3列出了引出一段外源dna進入克隆載體的大概步驟。 -
Baculovirus is a kind of double - stranded circle large dna virus, which has been developed as an environment sound pesticide and a powerful vector of foreign protein expression as well as a potential vector of gene therapy
桿狀病毒是一類雙鏈環狀dna病毒，作為生物防治殺蟲劑及高效外源蛋白表達載體得到廣泛研究。近來有許多學者致力於桿狀病毒作為基因治療載體的研究。 -
The purpose of this thesis was to clone two different group 3 lea genes. on one hand, we designed and synthesized a pair of degenerate primers according to the foreign reports. a 500bp dna fragment was obtained with pcr technique from the dna of highly drought - resistant china leymus
一方面，參照國外報道幾種植物的第三組lea中的hva1基因的相對保守的氨基酸序列，設計併合成一對兼并引物，提取抗旱、耐鹽性強的羊草的dna ，採用pcr技術，擴增出了495bp的一段基因片段。
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